| 荧光光谱法研究牛血清白蛋白与柠檬黄和日落黄的相互作用 |
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| 引用本文： | 刘志栋,韩德权,杨卫卫,李雪茹,邵淑双,孙庆申.荧光光谱法研究牛血清白蛋白与柠檬黄和日落黄的相互作用[J].食品科学,2014,35(9):128-131. |
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| 作者姓名： | 刘志栋 韩德权 杨卫卫 李雪茹 邵淑双 孙庆申 |
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| 作者单位： | 黑龙江大学生命科学学院，黑龙江省微生物高校重点实验室，教育部农业微生物工程中心，黑龙江 哈尔滨 150080 |
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| 基金项目： | 黑龙江省教育厅面上项目(11551360);国家自然科学基金青年科学基金项目(31000773) |
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| 摘 要： | 用紫外-可见光吸收光谱、荧光发射光谱法研究牛血清白蛋白与柠檬黄和日落黄之间的相互作用;用SternVolmer方程处理实验数据,得到310 K时的动态猝灭速率常数(KQ)分别为2.51×105 L/mol和1.42×105 L/mol;用Lineweaver-Burk双倒数函数方程处理实验数据,得到310 K时静态猝灭常数(KLB)依次为5.79×105 L/mol和5.56×105 L/mol;用lg((F0-F)/F)=lgK0+nlgρ处理实验数据,得到结合位点数(n)分别为0.556 95、0.640 56。并且两种色素在质量浓度为0~3μg/mL范围内其含量与荧光猝灭强度具有良好的线性关系。
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| 关 键 词： | 牛血清白蛋白 柠檬黄 日落黄 荧光猝灭 |
Interactions of Bovine Serum Albumin with Lemon Yellow and Sunset Yellow Studied by Fluorescence Spectroscopy |
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| LIU Zhi-dong,HAN De-quan,YANG Wei-wei,LI Xue-ru,SHAO Shu-shuang,SUN Qing-shen.Interactions of Bovine Serum Albumin with Lemon Yellow and Sunset Yellow Studied by Fluorescence Spectroscopy[J].Food Science,2014,35(9):128-131. |
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| Authors: | LIU Zhi-dong HAN De-quan YANG Wei-wei LI Xue-ru SHAO Shu-shuang SUN Qing-shen |
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| Affiliation: | Key Laboratory of Microbiology of Heilongjiang Province, Engineering Research Center of Agricultural Microbiology Technology,;
Ministry of Education, College of Life Science, Heilongjiang University, Harbin 150080, China |
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| Abstract: | Interactions between bovine serum albumin (BSA) and lemon yellow or sunset yellow were studied by UV-visible
absorption spectroscopy and fluorescence emission spectroscopy. The dynamic quenching rate constants at 310 K were
calculated according to Stern-Volmer equation to be 2.51 × 105 and 1.42 × 105 L/mol for lemon yellow and sunset yellow,
respectively. The static quenching constants at 310 K were calculated according to Lineweaver-Burk double-reciprocal
equation to be 5.79 × 105 and 5.56 × 105 L/mol, respectively. The number of binding sites at 310 K was calculated
according to lg((F0－F)/F)=lgK0+nlgρ to be 0.556 95 and 0.640 56, respectively. A good linear relationship was observed
between lemon yellow or sunset yellow concentration in the range of 0 – 3 μg/mL and fluorescence quenching intensity. |
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| Keywords: | bovine serum albumin lemon yellow sunset yellow fluorescence quenching |
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