| 聚合酶链式反应快速检测畜肉食品中鸭源性成分 |
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| 引用本文： | 史艳宇,刘金华,王莹,邴炜,华蕾,刘晓晖,王颖,周亮,王潇.聚合酶链式反应快速检测畜肉食品中鸭源性成分[J].食品科学,2014,35(10):163-165. |
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| 作者姓名： | 史艳宇 刘金华 王莹 邴炜 华蕾 刘晓晖 王颖 周亮 王潇 |
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| 作者单位： | 1.吉林省产品质量监督检验院，吉林 长春 130022；2.吉林出入境检验检疫局，吉林 长春 130062 |
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| 基金项目： | 国家质检总局科技计划项目(2012QK169) |
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| 摘 要： | 目的：建立一种快速、特异、灵敏的鸭源性成分检测方法。方法：以鸭细胞色素C氧化酶Ⅲ（COⅢ ）基因序列为靶位点设计引物，进行聚合酶链式反应扩增，建立鸭源性成分检测方法；以常见畜禽肉，包括羊肉、牛肉、鸡肉、鹅肉、猪肉、兔肉、马肉、鹿肉等参考动物物种进行特异性检测；以50 mg/kg羊肉DNA作为稀释液，对鸭肉DNA进行梯度稀释，检测灵敏度。结果：该方法能够有效的对鸭源性成分进行快速检测，具有较强的特异性，灵敏度较高（0.1 μg/kg），且羊肉成分的存在对鸭肉灵敏度检测没有影响，可以快速、准确检测畜肉食品中掺杂的鸭源性成分。
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| 关 键 词： | 鸭源性成分 细胞色素C氧化酶Ⅲ基因 聚合酶链式反应 检测 |
A Polymerase Chain Reaction Method for Rapid Detection of Duck-Derived Materials in Meat Products |
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| SHI Yan-yu,LIU Jin-hua,WANG Ying,BING Wei,HUA Lei,LIU Xiao-hui,WANG Ying,ZHOU Liang,WANG Xiao.A Polymerase Chain Reaction Method for Rapid Detection of Duck-Derived Materials in Meat Products[J].Food Science,2014,35(10):163-165. |
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| Authors: | SHI Yan-yu LIU Jin-hua WANG Ying BING Wei HUA Lei LIU Xiao-hui WANG Ying ZHOU Liang WANG Xiao |
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| Affiliation: | 1. Jilin Product Quality Supervision Inspection, Changchun 130022, China;
2. Jilin Entry-Exit Inspection and Quarantine Bureau, Changchun 130062, China |
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| Abstract: | This report describes a rapid polymerase chain reaction (PCR) method to detect duck-derived DNA by using
primers corresponding to the duck COⅢ gene. The method was duck specific, free from interference from lumb DNA (50
mg/kg). Its sensitivity was sufficient to detect as low as 0.1 μg/kg duck-derived materials. It can provide a rapid and accurate
way to identify duck-derived materials adulteration in meat products without interference of mutton DNA. |
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| Keywords: | duck-derived materials cytochrome oxidase Ⅲ (COⅢ ) gene polymerase chain reaction (PCR) detection |
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