SARP1在创伤性肌腱瘢痕中分布特点

目的 本文探讨分泌的凋亡相关蛋白基因SARP1在创伤后肌腱瘢痕形成与发展中基因分布特点与变化趋势以及在增生性瘢痕中所起的作用及意义.方法 收集标本,创伤后肌腱瘢痕增生12例,无瘢痕愈合12例,按伤后3、6、9、12个月不同时间分组编号.另设正常皮肤对照12例.作为研究对象,利用SARP1的基因特异片段制成寡核苷酸探针,与瘢痕组织切片和成纤维细胞爬片原位杂交,统计学处理后分析其变化规律.结果 早期创伤后肌腱瘢痕增生中SARP1基因的探针在组织细胞中有较强阳性的反应,而无瘢痕愈合则阳性反应较低,正常皮肤则更少.经对所有标本的观察,创伤后肌腱瘢痕增生3～6个月SARP1的表达无论阳性细胞的数量,还是反应的着色程度,明显强于9～12个月增生性瘢痕、无瘢痕愈合与正常皮肤.成纤维细胞爬片原位杂交结果 存在相同的结果 .结论 肌腱瘢痕增生与分泌的凋亡相关蛋白基因之间存在密切关系,而SARP1起到十分重要的作用.同时,调节该基因的表达,有望实现减轻肌腱瘢痕粘连.

Effect of gene expression of fibroblast secreted apoptosis-related protein 1(SARP1) on the hypertrophic scar of the tendon

Objective To investigate the effect of gene expression of fibroblast SARP1 on the formation and of hypertrophic scar in tendon after trauma.Methods Some special parts of cDNA of SARP1 was selected and made into oligonucleotide probe.12 cases of hypertrophic scar of tendon and 12 non-hypertrophic scar of tendon and 12 normal skin were used and these scar were taken on 3,6,9,12 months after trauma.Freezing slices and cultured fibroblasts were made to detect the expression of these genes by hybridization in si...