促血管生成素-2和血管内皮生长因子在结直肠癌组织中的表达

目的探讨促血管生成素-2（Ang-2）及血管内皮生长因子（VEGF）在结直肠癌组织及血液中的表达，研究其与肿瘤疾病分
期的关系。方法采用实时荧光定量PCR检测结直肠癌患者正常组织、癌旁组织及腺癌中Ang-2、Tie-2mRNA的表达，采用酶联
免疫竞争法检测Ang-2及VEGF在正常组织、癌旁组织及腺癌和外周血中的表达。结果癌组织Ang-2mRNA和Tie-2mRNA表
达与正常组织有明显差异（P<0.05），相关分析表明，2者表达存在着明显的相关（r=0.879，P=0.000）。癌组织Ang-2和VEGF含
量与正常组织有明显差异（P<0.05），与癌旁组织有明显差异（P<0.05）。癌外周血液Ang-2含量与正常对照外周血有明显差异
（P<0.05），与癌肠系膜血无明显差异（P>0.05）。肿瘤组织中Ang-2和VEGF含量与疾病分期明显相关，肿瘤患者显著高于正常
对照组（P<0.05），Dukes’ C期和D期显著高于A期和B期（P<0.05）。结论在大肠腺癌组织中，Ang-2、Tie-2及VEGF的过度表
达对肿瘤的血管生成和发展过程起着重要作用。

Expression of angiopoietin-2 and vascular endothelial growth factor in human colon cancer

Objective To investigate the association of the expressions of angiopoietin-2 (Ang-2), vascular endothelial growth
factor (VEGF) in colorectal cancer tissues with Dukes’ clinicopathological features. Methods Ang-2 and Tie-2 mRNA
expressions were detected in colorectal cancer tissues, adjacent tissues, and normal tissues by real time-PCR. Quantikine
immunoassays were used to measure the protein expressions of Ang-2 and VEGF in the tissues and serum samples. Results
Ang-2 and Tie-2 levels were significantly higher in the serum of the patients than in the normal tissues (P<0.05), and their
expressions were strongly correlated (r=0.879, P=0.000). Tumor tissue Ang-2 and VEGF levels were significantly higher than
their levels in the adjacent and normal tissues (P<0.05). In colorectal cancer patients, the peripheral blood level of Ang-2 was
significantly higher than that in healthy control subjects, and comparable with that in mesenteric blood (P>0.05). In Dukes’
stage C and D patients, serum Ang-2 and VEGF levels were significantly higher than those in patients in Dukes’ stage A and B
(P<0.05). Conclusion Ang-2 and VEGF overexpressions may play an important role in the occurrence and progression of
colorectal cancer.