脂联素基因多态性与新疆地区汉族2型糖尿病的相关性研究

目的研究脂联素(APN)基因45T/G单核苷酸多态性(SNPs)与新疆地区汉族人2型糖尿病(T2DM)的相关性。方法采用聚合酶链反应-限制性内切酶片段长度多态性技术(PCR-RFLP),比较新疆地区汉族人正常糖耐量组(NGT)和T2DM组APNSNP45基因型与等位基因分布频率差异;比较相同人群不同基因型和不同人群相同基因型T2DM相关体测与生化指标的差异。结果 T2DM组APNSNP45TG+GG基因型及G等位基因分布频率均高于NGT组(P﹤0.005);NGT组TG+GG基因型者血清甘油三酯(TG)、总胆固醇(CH)和低密度脂蛋白(LDL)水平显著高于TT基因型者(P﹤0.005),相同基因型人群之间,T2DM组收缩压(SBP),体重指数(BMI),腰臀比(WHR),空腹血糖(FBG),胰岛素抵抗指数(LOG(HOMA-IR)),TG,CH及LDL均高于NGT组(P﹤0.05);胰岛β细胞功能指数(LOG(HOMA-β))和高密度脂蛋白(HDL)水平均低于NGT组(P﹤0.005)。结论 APNSNP45T/G多态位点与新疆地区汉族T2DM相关,携G等位基因者发生T2DM的可能性增加,且G等位基因与汉族血脂代谢异常及胰岛素抵抗有关。

Association of 45T/G polymorphism adiponectin gene with type 2 diabetes in hans of the Xinjiang

OBJECTIVE To investigate the association adiponectin single nucleotide polymorphism(SNP)45T/G with the prevalence of Type 2 diabetes mellitus(T2DM)in Han people of the Xinjiang region of China. METHODS We performed a cross-sectional survey in a representative sample of 639 Han adults. The polymerase chain reaction–restriction fragment length polymorphism(PCR-RFLP)method was used to determine the distribution of allele and genotype frequency of the SNP45 T/G polymorphism(exon 2)in the adiponectin gene. Many anthropometric and biological indices were compared in different and same genotype carriers. RESULTS Compared with the normal glucose tolerance(NGT)group,the T2DM group exhibited a higher distribution of the TG + GG genotype and G allele frequency(P﹤0.005). Compared with SNP45 T carriers,in the NGT group,G carriers had higher levels of triglyceride(TG),total cholesterol(CH)and low-density lipoprotein(LDL)(P﹤0.005). In the people with same genetype,the T2DM group had lower levels of homeostasis model assessment of pancreatic β-cell function(HOMA-β)and high-density lipoprotein(HDL)(P﹤0.005),higher levels of systolic blood pressure(SBP),mass index(BMI),ratio of waist and Hip(WHR),fasting plasma glucose(FBG),HOMA of body insulin resistance(HOMA-IR),TG,CH,and LDL(P﹤0.05)than NGT group. CONCLUSION Adiponectin SNP 45 was positively correlated with the prevalence of T2DM in Han of Xinjiang. The G allele carriers may have associated with insulin resistance and hyperlipoidemia.