壳聚糖与神经干细胞生物相容性的研究

目的 探讨生物降解材料壳聚糖与神经干细胞的生物相容性。方法 取SD大鼠胚胎（孕14-16d)大脑皮层组织制成单细胞悬液在无血清培养液中进行培养，获得大量的神经干细胞，再将所获神经干细胞在不同条件下移植接种至壳聚糖膜上联合培养1周，在倒置显微镜下观察神经干细胞生长增殖情况及形态变化，并对其分别进行免疫组化染色，电镜观察，了解壳聚糖对神经干细胞生长，增殖，分化的影响。结果 在无血清联合培养条件下，神经干细胞仍然维持其原有的干细胞特性；在含血清的培养液中，神经干细胞能分化成多种形态的神经细胞，并且在壳聚糖膜上生长良好。结论 壳聚糖与神经干细胞具有良好的生物相容性。

Study of the Biocompatibility between Chitosan and Neural Stem Cells

Objective To explore the biocompatibility of biodegradable material of chitosan and neural stem cells.Methods Cells from the embryonic Sprague-Dawley rat(SD rat) cerebral cortices were mechanically dissociated and then cultured and expanded in the medium of DMEM/F 12,B27,and bFGF,these fast expanding and neurosphere-forming cells were cocultured with chitosan in the above medium and in the medium supplemented by 1% NBS (Newborn Bovine Serum,NBS) for one week.The proliferation and differentiation of these cells on the chitosan were observed by inverted microscope,and then these cells were stained by anti-nestin and other immunocytochemical procedures,to observe the influence of chitosan on these cells and examined by scanning electron microscope,to explore the biocompatibility of them.Results Neural stem cells were successfully cultured from embryonic SD rat cerebral cortices,and they formed typical floating neurospheres in the medium,and were stained by anti-nestin.When cocultured with chitosan in different conditions,neural stem cells were able to grow very well on the chitosan membrane and retain the characteristics of stem cells,and were able to further differentiate into neurons and neuroglia cells when the medium was supplemented with 1% NBS.Conclusions Neural stem cells can be cultured and isolated from embryonic SD rat cerebral cortices.Biocompatibility between chitosan and neural stem cells is very good.