p14ARF对人黑色素瘤细胞增殖的影响及其作用机理的初探

ARF(alternative reading frame)作为INK4a/ARF的β转录产物，能够稳定p53, 诱导细胞周期阻断或凋亡.利用高表达p14^ARF的人黑色素瘤细胞模型,探讨了ARF抑制细胞增殖的分子作用机理.研究发现p14^ARF高表达能将细胞周期阻断在G1和G2期, p53, p21^cip1和p27^kip1蛋白水平明显增强, 而p-ERK1/2,CyclinD1和CyclinE蛋白水平下降, 明显抑制细胞生长. 提示p14^ARF能通过ERK(extracellular signal-regulated kinase)信号通路相互协调作用抑制A375细胞增殖.

Effects of Overexpressing p14ARF on The Proliferation in Human Melanoma Cells

ARF can induce cell cycle arrest or apoptosis. By using the A375 cell overexpressing p14^ARF as a model, the molecular machanism of cell cycle arrest by ARF was studied. It was indicated that overexpressing p14^ARF resulted in G1-phase and G2-phase arrest. In the A375 cells overexpressing p14^ARF, the level of p53, p21^cip1 and p27^{kip1 was upregulated, but the protein lever of p-ERK1/2, Cyclin D1 and Cyclin E was inhibited. The results indicated that p14ARF induced the cell cycle arrest of A375 not only in a p53-dependent way, but also in coopration with the ERK signal transduction pathway.}