| 西安地区病毒性肝炎患者TTV DNA的检测及临床意义 |
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| 引用本文： | 傅恩清,白雪帆,潘蕾,李光玉,杨为松,王平忠.西安地区病毒性肝炎患者TTV DNA的检测及临床意义[J].第四军医大学学报,2000,21(7):824-826. |
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| 作者姓名： | 傅恩清 白雪帆 潘蕾 李光玉 杨为松 王平忠 |
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| 作者单位： | 第四军医大学唐都医院感染科,陕西,西安,710038 |
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| 摘 要： | 目的 调查西安地区不同类型肝炎患者输血传播病毒（ＴＴＶ）感染状况并探讨其临床意义。方法 在病毒ＯＲＦ１区设计内／外两对引物，采取ｎｅｓｔｅｄ－ＰＣＲ方法，检测病毒性肝炎患者１４７例和非病毒性肝炎患者１２８全血清中ＴＴＶ ＤＮＡ。结果 在肝炎组中，ＴＴＶ基因检测阳性率分别淡非甲非戊型肝炎６０．０％（９／１５），庚型肝炎５０．０％（６／１２），乙型肝炎２６．２％（２１／８０），丙型肝炎１１．１％（３／
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| 关 键 词： | 输血传播病毒 病毒性肝炎 套式聚合敏链反应 |
Detection of transfusion transmitted virus DNA in patients with viral hepatitis in Xi'an by nested-PCR |
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| FU En-Qing,BAI Xue-Fan,PAN Lei,LI Guang-Yu,YANG Wei-Song,WANG Ping-Zhong.Detection of transfusion transmitted virus DNA in patients with viral hepatitis in Xi'an by nested-PCR[J].Journal of the Fourth Military Medical University,2000,21(7):824-826. |
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| Authors: | FU En-Qing BAI Xue-Fan PAN Lei LI Guang-Yu YANG Wei-Song WANG Ping-Zhong |
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| Abstract: | AIM To detect transfusion transmitted virus (TTV) DNA from patients with viral hepatitis and investigate TTV infection in Xi'an on clinical background. METHODS A nested polymerase chain reaction (PCR) assay with two pairs of primers complemented with ORF1 of TTV genome was established to detect TTV DNA in the serum samples from 147 patients with viral hepatitis and 128 patients without hepatitis in Xi'an. RESULTS TTV DNA was detected in sera from 9 of 15 (60.0%) non A-non E hepatitis, 6 of 12 (50.0%) hepatitis G, 21 of 80 (26.2%) hepatitis B, 3 of 27 ( 11.1% ) hepatitis C, 0 of 6 (0%) hepatitis A, 0 of 2 (0%) hepatitis A companied with hepatitis C, 3 of 5 hepatitis B companied with hepatitis C, 3 of 128 (2.3%) non hepatitis patients in Xi'an. CONCLUSION TTV infection is predominant in patients with hepatitis B, C, G and non A to non E. TTV may have pathogenisity to human. |
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| Keywords: | transfusion transmitted virus viral hepatitis nested-PCR |
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