|
|||||
|
|
| 小刺猴头多糖联合5-氟尿嘧啶对Hela细胞的抑制作用 | |
| 引用本文： | 王雪,宋慧,刘小腊,苏玲,姚旭.小刺猴头多糖联合5-氟尿嘧啶对Hela细胞的抑制作用[J].中国生化药物杂志,2012,33(5):625-628. |
| 作者姓名： | 王雪 宋慧 刘小腊 苏玲 姚旭 |
| 作者单位： | 1. 吉林农业大学生命科学学院,吉林长春130118;大连珍奥药业有限公司,辽宁大连116620 2. 吉林农业大学生命科学学院,吉林长春130118;食药用菌教育部工程研究中心,吉林长春130118 3. 吉林农业大学生命科学学院,吉林长春,130118 |
| 摘 要： | 目的研究体外单独应用小刺猴头菌多糖及联合5-氟尿嘧啶(5-Fu)对宫颈癌Hela细胞的增殖抑制作用。方法水提醇沉法得到小刺猴头液体深层发酵浸膏多个糖组分,经MTT筛选获得小刺猴头菌多糖最优组分Hep2。设置空白、Hep2、Hep2+5-Fu、5-Fu组进行MTT实验及细胞周期检测。结果 Hep2单独给药及联合给药都能抑制Hela细胞增殖;Hep2组出现G1期阻滞现象;联合给药组发生了S期和G2/M期阻滞。结论 Hep2可以明显抑制Hela细胞的增殖,并且与5-Fu联合使用对Hela细胞产生协同抑制作用。 |
| 关 键 词： | 小刺猴头多糖 液体深层发酵浸膏 5-氟尿嘧啶 细胞周期 |
A polysaccharide from H.caput-medusae(Bull.:Fr.) Pers combines with 5-Fluorourail against Hela cell lines |
|
| WANG Xue , SONG Hui , LIU Xiao-la , SU Ling , YAO Xu.A polysaccharide from H.caput-medusae(Bull.:Fr.) Pers combines with 5-Fluorourail against Hela cell lines[J].Chinese Journal of Biochemical Pharmaceutics,2012,33(5):625-628. | |
| Authors: | WANG Xue SONG Hui LIU Xiao-la SU Ling YAO Xu |
| Affiliation: | 1(1.School of Life Science,Jilin Agricultural University,Changchun 130118,China;2.Zhen-aoPharmaceutical Company Limited,Dalian 116620,China;3.Edible and Medicinal Fungi EngineeringResearch Center of Ministry of Education,Changchun 130118,China) |
| Abstract: | Purpose To investigate the inhibiting effect of polysaccharides from H.caput-medusae(Bull.:Fr.) Pers alone or combined with 5-Fu added in vitro on the growth of Hela.Methods Polysaccharides of H.caput-medusae(Bull.:Fr.) Pers were obtained through water extraction and alcohol precipitation,and Hep2 was screened as the best constituent through MTT assay.The cells were divided into 4 groups,the control group and other three groups which were treated with Hep2,Hep2+5-Fu and 5-Fu,to be tested by MTT and Cell Cycle.Results Both Hep2 and Hep2+5-Fu inhibited the growth of Hela by arrsetting it at G1 phase,S phase and G2/M phase arrest respectively.Conclusion Hep2 could significantly inhibit the growth of Hela in vitro and had a synergistic inhibitory effect when combined with 5-Fu. |
| Keywords: | polysaccharide from H caput-medusae(Bull :Fr ) Pers liquid submerged fermentation extract fluorouracil cell recycle |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |
