口腔感染白色念珠菌的基因分型及药敏分析

目的 研究口腔黏膜念珠菌病患者白色念珠菌的基因分型及药敏试验分析.方法 采用聚合酶链反应（PCR）对78株口腔白色念珠菌基因分型,用纸片扩散（K-B）法进行药敏试验,测定白色念珠菌不同基因型对两种临床常用抗真菌药物的敏感性,采用SPSS16.0软件包对数据进行统计分析.结果 口腔念珠菌病患者唾液中分离的白色念珠菌A型占57.7％,B型占26.9％,C型占15.4％.药敏试验结果为：78株白色念珠菌对氟康唑敏感的占89.7％,对伏立康唑敏感的占98.7％,对伏立康唑敏感率高于氟康唑（P＜0.05）.结论 引起口腔黏膜念珠菌病的白色念珠菌基因呈多态性,且不同基因型对抗真菌药物的敏感性存在较大差异,基因分型对口腔念珠菌病的诊断及合理用药有重要意义.

Genotyping and Antifungal Susceptibility Test of Candida Albicans from Patients with Candidosis

Objective To investigate the genotypes and antifungal susceptibility of candida albicans from patients with candidosis.Methods Polymerase chain reaction （PCR） was adopted to analyze78 Candida albicans which were isolated from patients with candidosis,Kirby-Bauer （K-B） paper dispersion method was carried out for antifungal susceptibility test.SPSS16.0 software package was used for data analyzing.Results 78 strains of Candida albicans were divided into three types,genotype A were 57.7%,genotype B were 26.9%,genotype C were 15.4% respectively.The susceptibility rate of Candida albicans to fluconazol was 89.7%,while the susceptibility rate to voriconazole was 98.7%.The susceptibility rate to voriconazole was higher than to flucoriazol （P 〈 0.05）.Conclusion The genotype of Candida albicans from patients with candidosis is multiple.The susceptibility rate of Candida albicans to antifungal drugs is different according to their different genotypes.Genotyping is important to candidosis diagnosis and rational use of antifungal drugs.