An experimental study on nerve regeneration and spinal neurons after CO2 laser anastomosis of rat sciatic nerve |
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| Authors: | LIU Wei-ping ZHANG xiang WANG Yan-gang FEI Zhou CHEN Yi-jun FU Luo-an LIANG Jing-wen DUAN Xin-min YANG Ji-qing |
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| Affiliation: | Department of Neurosurgery, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China Department of Neurosurgery, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China Department of Neurosurgery, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China Department of Neurosurgery, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China Department of Neurosurgery, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China Department of Neurosurgery, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China Department of Neurosurgery, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China Department of Physiology, Fourth Military Medical University, Xi'an 710032, China Department of Physiology, Fourth Military Medical University, Xi'an 710032, China |
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| Abstract: | Objective:Manymethodshavebeenusedinan attemptto sealtheepineuriumandto preventaxonaloutgrowth.Inthisstudy,theratsciaticnerveswererepairedwithCO 2 laser,thenerveregenerationandthemorphologyof spinalante-riorhornneuronswereinvestigated. Methods:Seventy-twomaleSprague-Dawleyratswererandomlydivided into6groupsof12rats.Theanimalsweredesignedto observetheelectrophysiology,thehistopathologyandthemorphology of spinalanteriorhornneurons.Oneof theratsciaticnerveanastomosedwithCO 2 laser,thecontralateralnervewasrecon-structedby microsuturetechnique.At2,4,6,8weekspostoperatively,neuromuscularfunctions,theregenerationof axons andneuronswereevaluatedby theelectro-physiologicalandhistopathologicalstudies.Theratswerekilledat4,6weeks postoperatively.Results:Therecoveryof toespreadandmyodynamiainlasergroupswasbetterthanthatinsuturegroups(P<0.05).Thelatencyof footwithdrawcausedby radiateheatandneuromuscularconductionvelocityinlasergroupswere fasterthanthatin suturegroups(P<0.05).Thedensityof nervefibers,percentageof axonspassingthroughanastomotic areaandnumbersof neuronswerebetterinlasergroupsthaninsuturegroups.At8weekspostoperatively,thefirstgrade dendritesof anteriorhornneuronsgrewwell.Theirdiameter,length,volumeandtotalvolumeweremuchhigherthanthat in controlgroup.(P<0.05,P<0.01).Conclu sion:CO 2 laserrepairingwas effectivein promotingtheregenerationandthe recoveryof sciaticnervesinitsearlypost-traumastage.Inaddition,laserrepairingwasfoundto reduceregeneratingax-onsmisdirectionandformingneuroma. |
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| Keywords: | CO2 laser sciatic nerves nerve function nerve regeneration spinal neurons |
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