Impact of dietary FA and energy restriction on plasma leptin and ob gene expression in mice |
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Authors: | Gregersen Søren Thomsen Janus L Jeppesen Per B Alstrup Kirsten K Brock Birgitte Pedersen Steen B Kristensen Kurt Hermansen Kjeld |
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Affiliation: | (1) Department of Endocrinology and Metabolism, Aarhus University Hospital, Tage-Hansensgade 2, 8000 Aarhus C, Denmark |
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Abstract: | The aim of the present study was to elucidate whether the qualitative compositiion of dietary fat influences plasma leptin
and adipose tissue ob gene expression differentially. Two high-fat diets and a diet rich in carbohydrate were each administered
both ad libitum and with a 25% energy restriction. The high-fat diets contained 58 energy percent as either monounsaturated FA (MUFA) or
saturated FA (SAFA), whereas the carbohydrate-rich diet (CH) contained 7 energy percent as fat. We aimed at obtaining the
same final weight for the animals in the ad libitum group as in the energy-restricted groups. This goal was reached at he same time (days 22–24) for all groups except for the
ad libitum animals fed on saturated fat (day 36). The plasma leptin concentrations on ad libitum CH and MUFA diets did not differ significantly (24.3 ± 2.1 and 34.7 ± 6.7 ng/mL, respectively) whereas the saturated fat
diet caused a lower concentration (13.9 ± 1.9 ng/mL; P < 0.05). Interestingly, no differences in plasma leptin levels between groups were seen in the energy-restricted groups (mean
8.0 + 1.0 ng/mL). The type of diet did not alter the ob gene expression in intraabdominal white adipose tissue; however, a
lower expression level was found in the energy-restricted groups. The percentage of body fat in the three ad libitum fed groups did not differ (23 ± 1%). Thus, short-term administration of a diet rich in SAFA suppresses circulating leptin
levels without altering the adipose tissue ob gene expression. This indicates that saturated fat may alter protein handling
by adipose tissue or the whole body clearance of leptin. |
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