| 修饰血红素提高血红蛋白类过氧化物酶活性 |
| |
| 引用本文： | 凌辉生,李阳,封琳,李任强. 修饰血红素提高血红蛋白类过氧化物酶活性[J]. 生物加工过程, 2011, 9(2): 68-71. DOI: 10.3969/j.issn.1672-3678.2011.02.014 |
| |
| 作者姓名： | 凌辉生 李阳 封琳 李任强 |
| |
| 作者单位： | 暨南大学,生物工程学系,广州,510632 |
| |
| 基金项目： | 暨南大学自然科学基金资助项目(109057640073) |
| |
| 摘 要： | 利用苯酚或对羟基联苯对血红蛋白的血红素辅基进行化学修饰,将修饰后的血红素与脱辅基血红蛋白进行重组得到新的血红蛋白。以光吸收扫描分析修饰血红素和重组血红蛋白,证明新的重组血红蛋白构建成功。酶活力测定表明,修饰血红素得到的重组血红蛋白的类过氧化物酶活性都比天然血红蛋白的酶活力高,用对羟基联苯修饰血红素得到的重组血红蛋白的酶活提高明显,约是天然血红蛋白的1.6倍。
|
| 关 键 词： | 化学修饰 血红素 血红蛋白 过氧化物酶 酶活力 |
Improving peroxidase-like activity of hemoglobin by recombining hemoglobin using chemically modified heme |
| |
| LING Huisheng,LI Yang,FENG Lin,LI Renqiang. Improving peroxidase-like activity of hemoglobin by recombining hemoglobin using chemically modified heme[J]. Chinese Journal of Bioprocess Engineering, 2011, 9(2): 68-71. DOI: 10.3969/j.issn.1672-3678.2011.02.014 |
| |
| Authors: | LING Huisheng LI Yang FENG Lin LI Renqiang |
| |
| Affiliation: | LING Huisheng,LI Yang,FENG Lin,LI Renqiang(Department of Biotechnology,Jinan University,Guangzhou 510632,China) |
| |
| Abstract: | The phenol or 4-biphenylol was used to modify the heme.The modified hemin group was recombined with apo-hemoglobin to prepare novel artificial hemoglobin.Absorption spectral scan demonstrated that the artificial recombined hemoglobins with modified heme were obtained.Experimental results showed that the peroxidase-like activity of modified hemoglobins was enhanced compared with that of native hemoglobin.And the enzymatic activity of modified hemoglobin with 4-biphenylol modified heme was as about 1.6 folds ... |
| |
| Keywords: | chemical modification heme hemoglobin peroxidase enzymatic activity |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
