| 东北龙胆花芽蛋白质双向电泳条件的建立 |
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| 引用本文： | 朱宏,于丽杰.东北龙胆花芽蛋白质双向电泳条件的建立[J].东北林业大学学报,2009,37(2). |
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| 作者姓名： | 朱宏 于丽杰 |
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| 作者单位： | 哈尔滨师范大学,哈尔滨,150080 |
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| 摘 要： | 初步建立了东北龙胆(Gentiana manshuica Kitagawa)花芽总蛋白质提取方法,以及东北龙胆花芽蛋白质组研究的双向电泳技术.从样品制备及溶解等方面进行了优化,解决了东北龙胆花芽蛋白质提取中色素、多糖、酚类物质等对双向电泳的干扰,确定了一种有效的蛋白溶解液(7 mol/L尿素,2 mol/L硫脲,4% CHAPS,40 mmol/L DTT,1%蛋白酶抑制剂混合物,2%的两性电解质pH=3～10).同时,对电泳其它环节也进行了优化,经考马斯亮兰染色后,可分辨蛋白质斑点数约1 000个.获得了重复性好、分辨率高的蛋白质双向电泳图谱.
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| 关 键 词： | 东北龙胆 花芽 蛋白质组 双向电泳 |
Establishment of A Two Dimensional Electrophoresis Protocol for Protein of Gentiana manshurica Flower Bud |
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| Zhu Hong,Yu Lijie.Establishment of A Two Dimensional Electrophoresis Protocol for Protein of Gentiana manshurica Flower Bud[J].Journal of Northeast Forestry University,2009,37(2). |
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| Authors: | Zhu Hong Yu Lijie |
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| Affiliation: | Zhu Hong,Yu Lijie(Harbin Normal University,Harbin 150080,P.R.China) |
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| Abstract: | An extraction method of total protein from Gentiana manshurica Kitag.flower bud and the technology of two-dimensional electrophoresis of flower bud proteome were initially established in order to raise resolution and repeatability.The preparation and dissolution of samples were optimized,which solved the interference of pigment,polysaccharide,and phenol materials to the two-dimensional electrophoresis disturbance in the process of extraction of G.manshurica flower bud.A kind of effective protein dissolution... |
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| Keywords: | Gentiana manshurica Proteome Flower buds Two-dimensional electrophoresis |
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