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| 淋巴瘤细胞转染GM-CSF 基因后生物学特性的变化 | |
| 引用本文： | 黄明宜,高全立,马保根,宋永平,张叔人.淋巴瘤细胞转染GM-CSF 基因后生物学特性的变化[J].郑州大学学报(医学版),2002,37(4):480-482. |
| 作者姓名： | 黄明宜 高全立 马保根 宋永平 张叔人 |
| 作者单位： | 1. 河南职工医学院临床部,郑州,450003 2. 河南省血液病研究所,河南省肿瘤医院血液科,郑州,450003 3. 中国医学科学院肿瘤研究所免疫室,北京,100021 |
| 摘 要： | 目的：制备高表达GM－CSF的淋巴瘤细胞系，观察淋巴瘤细胞转染GM－CSF基因后生物学特性的改变。方法：将小鼠GF－CSF真核表达质粒用电穿孔法导入小鼠淋巴瘤细胞系RMA，有限稀释法制备单个细胞克隆，经RT－PCR，骨髓祖细胞增殖实验和集落形成实验筛选相对高表达GM－CSF的RMA克隆，并观察其生物学特性的改变。结果：获得了高表达GM－CS的RMA细胞系,其同基因动物体内致瘤性降低。结论：淋巴瘤细胞系RMA转染GM－CSF基因后致瘤性降低。 |
| 关 键 词： | GM－CSF基因 淋巴瘤 免疫治疗 |
| 修稿时间： | 2001年12月20 |
Changes of biological character of lymphoma cell transfected with GM-CSF gene |
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| HUANG Mingyi ,GAO Quanli ,MA Baogen ,SONG Yongping ,ZHANG Shuren Clinical.Changes of biological character of lymphoma cell transfected with GM-CSF gene[J].Journal of Zhengzhou University: Med Sci,2002,37(4):480-482. | |
| Authors: | HUANG Mingyi GAO Quanli MA Baogen SONG Yongping ZHANG Shuren Clinical |
| Affiliation: | HUANG Mingyi 1),GAO Quanli 2),MA Baogen 2),SONG Yongping 2),ZHANG Shuren 3) 1)Clinical Department,Henan Officers and Workers' Medical College,Zhengzhou 450003 2)Henan Province Tumor Hospital,Zhengzhou 450003 3)Department of Immun |
| Abstract: | Aim: To select high GM-CSF expressing mouse leukemia cell lineage RMA after transfected with GM-CSF gene. Methods: Eukaryotic GM-CSF expressing plasmid was transfected into RMA cells by electroporation. After screening by G418 and cloning by limited dilution, a relative GM-CSF high expressing cell clone was selected by RT-PCR, hematopoietic progenitor cell proliferative assay and hematopoietic progenitor cell colony formation assay. Results: A GM-CSF high expressing cell lineage RMA-GM was selected whose ability of tumor formation was lower than RMA. Conclusion: The in vivo growth ability of lymphoma cell line RMA is suppressed after transfected with GM-CSF gene. |
| Keywords: | GM-CSF gene lymphoma immunotherapy |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |
