辛伐他汀对烟雾吸入性肺损伤大鼠炎性因子及氧化应激反应的影响

目的:研究辛伐他汀对烟雾吸入性肺损伤大鼠炎性因子及氧化应激反应的影响。方法:选取60只清洁级SD大鼠,将其按照随机抽签法分成正常组、盐水组以及辛伐他汀组,每组各20只。盐水组与辛伐他汀组大鼠均制备发烟罐烟雾吸入性肺损伤模型,建模成功后30 min,辛伐他汀组大鼠予以50 mg/kg剂量的辛伐他汀灌胃,盐水组则予以等量的生理盐水灌胃,正常大鼠予以正常饲养处理。采用酶联免疫法检测血清、肺泡灌洗液中炎症因子包括白细胞介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)]及氧化应激反应指标包括超氧化物歧化酶(SOD)、丙二醛(MDA)]水平。结果:盐水组、辛伐他汀组大鼠血清、肺泡灌洗液中IL-6、TNF-α水平均高于正常组,且辛伐他汀组大鼠上述各项指标低于盐水组(均P<0.05)。盐水组、辛伐他汀组大鼠血清、肺泡灌洗液中SOD水平低于正常组,辛伐他汀组明显高于盐水组(均P<0.05),盐水组、辛伐他汀组大鼠血清、肺泡灌洗液中MDA水平高于正常组,辛伐他汀组明显低于盐水组(均P<0.05)。结论:辛伐他汀对烟雾吸入性肺损伤大鼠的炎性因子具有明显的改善作用,且有利于减轻大鼠的氧化应激反应程度。

Effects of Simvastatin on Inflammatory Factors and Oxidative Stress in Rats with Smoke Inhalation Lung Injury

Objective: To study the effects of simvastatin on inflammatory factors and oxidative stress in rats with smoke inhalation lung injury. Methods: 60 SD rats of clean grade were selected, and they were divided into normal group, saline group and simvastatin group by random lottery, 20 rats in each group. Rats in saline group and simvastatin group were used to establish smoke inhalation lung injury model from smoking canisters. 30 minutes after successful modeling, rats in the simvastatin group were given 50 mg/kg of simvastatin by gavage, and rats in the saline group were given the same amount of normal saline by gavage, and normal rats were fed and treated normally. The levels of inflammatory factors(including interleukin-6(IL-6), tumor necrosis factor-α(TNF-α) and oxidative stress response indicators(including superoxide dismutase(SOD) and malondialdehyde(MDA) in serum and alveolar lavage fluid were detected by enzyme-linked immunoassay. Results: The expression levels of IL-6 and TNF-α in serum and alveolar lavage fluid of rats in the saline group and simvastatin group were higher than those in the normal group, and the above indicators in the simvastatin group were lower than those in the saline group(all P<0.05). The level of SOD in serum and alveolar lavage fluid of rats in the saline group and simvastatin group were lower than those in the normal group, while that in the simvastatin group was significantly higher than that in the saline group(all P<0.05). The level of MDA in serum and alveolar lavage fluid of rats in the saline group and simvastatin group were higher than that in the normal group, while that in the simvastatin group was significantly lower than that in the saline group(all P<0.05).Conclusion: Simvastatin can significantly improve the inflammatory factors in rats with smoke inhalation lung injury, and it can conducive to reducing the degree of oxidative stress in rats.