Cyclospora cayetanensis: oocyst characteristics and excystation |
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Authors: | Siripanth Chutatip Phraevanich Rangson Suphadtanaphongs Waraporn Thima Niramol Radomyos Prayong |
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Affiliation: | Department of Protozoology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand. tmcsr@mahidol.ac.th |
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Abstract: | In Thailand in 1999-2000, Cyclospora oocysts from two HIV-infected patients and one patient with prolonged diarrhea were detected by formalin-ether concentration technique. Sporulation was performed by mixing stool samples in 2.5% potassium dichromate solution, sporulated oocysts were then treated with various solutions before mechanical rupturing in order to establish excystation, fewer than 10% of the sporulated oocysts could be excysted. Our techniques provided more details of the characteristic appearance of sporocysts and sporozoites within the oocysts (DMSO-modified acid-fast technique with our modification). |
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