施CO2时培养基糖浓度对文心兰试管苗生长的影响

 探讨了培养室内施CO2条件下，培养基中不同蔗糖浓度(30、20、10、5、2．5和0 g·L )对文心兰试管苗生长的影响．结果表明：无糖培养基中的试管苗可以以光合自养的方式正常生长，但生长速度略低；提高培养基糖浓度可以显著促进试管苗的生长，30 g·L (常规培养条件的糖浓度)与20 g·L 处理相比，株高、叶长、叶幅、根数、总鲜样质量和总干样质量等主要生长指标无明显差异，说明文心兰试管苗在施CO₂培养时，培养基中的糖仍是必要的，但可以适当减少用量。

Effect of Sucrose Concentration on the Growth of Oncidium Explants in Vitro under CO2 Enrichment

Oncidium aloha ‘1wan ga’ in vitro shoots were used as plan t materials for experiments to study the efect of difierent sucrose concentrations (30，20，10，5，2．5，0 g·L )in the culture medium on the growth 0f the in vitro sho ts under CO，enrichment conditions． The results showed that the in vitro shoots had photoau totrophic capability． Under CO2 enrichm ent conditions， in vitro shots being cultivated in the sugar-free culturemed ium could grow normally by photoautotrophic capab ility，but a bit slowly． Increas ing the sucrose concentration in the culture medium could significan tly promote the growth of the in vitro shots of Oncidi． Under CO2 enrichment condition． in vitro sho ts grew be tter in the sugar culture medium than in the sugar-free culture medium ．Compared th the sucrose concentration (30 g·L )un der normal culture conditions ，when the sucrose concen tration dropped to 20 g·L|。． there was not significan t difference be tween the tw o concentration treatments in the main growth indices of the in vitro shots including plant height， length of leaves， range of leaves， number of roots． total fresh mas s an d dry matter mass． The above results indicate that under C02 enrichm ent cul ture conditions． the existence of sucrose in the culture medium is still necessary for Oncidium ，but compared with normal culture methods． it can partly reduce the amount of sucrose used in the culture medium ．