| 两种光度法测定灵芝多糖含量比较 |
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| 引用本文： | 蔡晓.两种光度法测定灵芝多糖含量比较[J].预防医学情报杂志,2012,28(3):236-238. |
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| 作者姓名： | 蔡晓 |
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| 作者单位： | 四川省疾病预防控制中心,成都,610041 |
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| 摘 要： | 目的比较灵芝及灵芝提取物和相关产品中多糖含量测定2种方法的差异。方法采用苯酚-硫酸法,蒽酮-硫酸法比色测定灵芝多糖含量。结果该2种方法测定实验结果基本一致,标准葡聚糖液在0.005～0.1 mg/ml浓度范围内线性关系良好,相关系数均大于0.999 4。结论该2种方法测定结果准确可靠,可用于灵芝多糖的含量测定。
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| 关 键 词： | 灵芝多糖 苯酚—硫酸法 蒽酮—硫酸法 |
Two Spectrophotometric Methods for Ganoderma Lucidum Polysaccharide Determination |
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| CAI Xiao.Two Spectrophotometric Methods for Ganoderma Lucidum Polysaccharide Determination[J].Journal of Preventive Medicine Information,2012,28(3):236-238. |
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| Authors: | CAI Xiao |
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| Affiliation: | CAI Xiao Sichuan Center for Disease Control and Prevention,Chengdu 610041,Sichuan Province,China. |
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| Abstract: | Objective To compare the difference of the two spectrophotometric methods for the polysaccharide determination of ganoderma lucidum and ganoderma lucidum eatract.Methods The content of ganoderma lucidum polysaccharide was measured by phenol-sulfuric acid and anthrone-sulfuric acid spectrophotometric methods.Results There were no significant difference between the results of the two methods.The linear relationship of standard solution between 0.005 mg/ml and 0.1 mg/ml was good,and correlation coefficient were both above 0.9994.Conclusion The two methods can be used to determine the content of ganoderma lucidum polysaccharide accurately. |
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| Keywords: | ganoderma lucidum polysaccharide phenol-sulfuric acid method anthrone-sulfuric acid method |
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