微生物间歇发酵生产1,3-丙二醇过程辨识与优化

以肺炎杆菌转化甘油生成1，3-丙二醇（1，3-PD）的过程为研究体系，根据微生物间歇培养过程的特征及动态行为，建立了简化的间歇发酵过程的非线性动力系统及其参数辨识模型，论述了动力系统与辨识问题解的存在性，并求得最优辨识参数．以初始底物浓度、菌种浓度及发酵时间为控制变量，以1，3-PD的生产强度为性能指标，建立了非线性系统的最优控制模型，针对实际算例求得最优解，为1，3-PD的产业化生产设计提供理论指导．

Parameter identification and optimization of process for bio-dissimilation of glycerol to 1,3-propanediol in batch culture

Using the process of forming 1,3-propanediol by transforming glycerol using Klebsiella pneumoniae as study system, a simplified mathematical model of the nonlinear dynamic system is developed according to the cell growth kinetics of multiple-inhibitions, the metabolic overflow kinetics of substrate consumption and product formation. The identification model for this system is also established. The existence of the solution for the nonlinear dynamic system is discussed and the identifiability of the parameters is proved. Finally, the optimal control problem of batch fermentations is obtained by using the volumetric productivity of 1,3-propanediol as the objective function and the initial concentrations of glycerol and biomass and fermentation time as the control variables. The results can provide the theoretical instruction for production design of 1,3-propanediol.