| T细胞免疫BALB/c小鼠诱导调节性体液免疫应答的研究 |
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| 引用本文： | 刘相国,王炜,鲁琰,高晓明.T细胞免疫BALB/c小鼠诱导调节性体液免疫应答的研究[J].中国免疫学杂志,2002,18(9):604-607. |
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| 作者姓名： | 刘相国 王炜 鲁琰 高晓明 |
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| 作者单位： | 1. 北京大学医学部免疫学系,北京,100083
2. 兰州生物制品所,兰州,730046 |
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| 摘 要： | 目的 :研究T细胞免疫后正常小鼠的调节性体液免疫应答。方法 :用经γ射线照射的体外扩增的活化OVA特异T细胞克隆免疫BALB c小鼠 ,FACS法分析血清中抗T细胞抗体水平 ,免疫共沉淀法分析靶抗原。结果 :T细胞免疫能诱导BALB c小鼠产生抑制T细胞增殖的抗T细胞抗体 ,这种抗体不是多克隆活化的结果 ,其靶抗原可能是T细胞上 93、87、5 5和 4 5kD的蛋白。结论 :T细胞免疫能诱导正常小鼠产生调节性体液免疫应答
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| 关 键 词： | T细胞免疫 体液免疫应答 免疫共沉淀 |
| 文章编号： | 1000-484X(2002)09-0604-04 |
T cell vaccination induces regulatory humoral immune response in BALB/c mice |
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| LIU Xiang Guo,WANG Wei ,LU Yan et al.T cell vaccination induces regulatory humoral immune response in BALB/c mice[J].Chinese Journal of Immunology,2002,18(9):604-607. |
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| Authors: | LIU Xiang Guo WANG Wei LU Yan |
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| Affiliation: | LIU Xiang Guo,WANG Wei *,LU Yan et al.Department of Immunology,Health Sciences Center,Peking University,Beijing 100083.*Lanzhou Institute of Biological Products,Lanzhou 730046 |
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| Abstract: | Objective:To investigate regulatory humoral immune response induced by T cell vaccination Methods:BALB/c mice were vaccinated with activated OVA specific T cell clones cultured in vitro, FACS assay was used to examine the anti T cell antibody level, and the target antigens of T cell were detected by immunoprecipitation assay Results:T cell vaccination could induce regulatory anti T cell antibodies which were not induced by polyclonal activation of lymphocytes, the target antigens of T cell were likely four proteins whose molecular weights were 93, 87, 55, 45 kD respectively Conclusion:Regulatory humoral immune response can be induced by T cell vaccination in normal mice |
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| Keywords: | Regulatory humoral immune response T cell vaccination Immunoprecipitation |
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