| 无精症和少精子症患者的细胞遗传学和分子遗传学检测 |
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| 引用本文： | 黄海龙,徐两蒲,李英,扶梅妹,林娜,王林铄,康跃凡,陈永艳,许金榜.无精症和少精子症患者的细胞遗传学和分子遗传学检测[J].中国妇幼保健,2011,26(30):4748-4750. |
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| 作者姓名： | 黄海龙 徐两蒲 李英 扶梅妹 林娜 王林铄 康跃凡 陈永艳 许金榜 |
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| 作者单位： | 福建省妇幼保健院,福建福州,350001 |
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| 基金项目： | 福建省自然科学基金资助项目，福建省卫生厅青年科研基金资助项目，福建省卫生厅青年科研资助项目 |
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| 摘 要： | 目的:对无精症和少精子症患者进行外周血染色体及Y染色体微缺失检测,探讨生精功能障碍的遗传学机制,为临床治疗和遗传咨询提供参考。方法:运用细胞遗传学核型分析技术和多重PCR技术对133例生精功能障碍患者进行染色体核型分析和Y染色体AZF因子扩增,并以40例已生育男性作为对照组。结果:实验组中61例无精子症患者中,细胞遗传学核型数量异常13例,异常发生率21.31%,同时发现AZF微缺失6例,异常发生率9.84%;72例少精患者细胞遗传学核型异常11例,异常发生率15.28%,同时发现AZF微缺失7例,异常发生率9.72%。40例对照组Y染色体核型和AZF位点无缺失。结论:染色体异常和AZF的缺失是引起男性无精子和少精子并造成男性不育的重要原因之一,对男性不育人群进行细胞遗传学核型分析和AZF检测十分必要。
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| 关 键 词： | 无精子症 少精子症 核型分析 无精子因子 |
Cytogenetic and molecular genetic test among patients with azoospermia and oligozoospermia |
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| HUANG Hai-Long,XU Liang-Pu,LI Ying et al.Fujian Maternal , Child Health Hospital,Fuzhou ,Fujian,China.Cytogenetic and molecular genetic test among patients with azoospermia and oligozoospermia[J].Maternal and Child Health Care of China,2011,26(30):4748-4750. |
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| Authors: | HUANG Hai-Long XU Liang-Pu LI Ying Fujian Maternal Child Health Hospital Fuzhou Fujian China |
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| Affiliation: | HUANG Hai-Long,XU Liang-Pu,LI Ying et al.Fujian Maternal and Child Health Hospital,Fuzhou 350001,Fujian,China |
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| Abstract: | Objective:To carry out chromosome and Y chromosome microdeletion test in peripheral blood of patients with azoospermia and oligozoospermia,explore genetic mechanism of spermatogenic arrest,provide a reference for clinical treatment and genetic consultation. Methods:Cytogenetic karyotype analysis technique and multiplex polymerase chain reaction(PCR) technique were used for chromosomal karyotype analysis and azoospermia factor(AZF) amplification of Y chromosome among 133 patients with spermatogenic arrest,40... |
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| Keywords: | Azoospermia Oligozoospermia Karyotype analysis Azoospermia factor |
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