| 血清剥夺对滋养细胞生物行为的影响及其机制研究 |
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| 引用本文： | 周晓宇,刘霞,金小英,彭华,钟少平,邹丽.血清剥夺对滋养细胞生物行为的影响及其机制研究[J].中国妇幼保健,2011,26(30):4752-4754. |
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| 作者姓名： | 周晓宇 刘霞 金小英 彭华 钟少平 邹丽 |
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| 作者单位： | 1. 嘉兴学院附属妇幼保健院产科,浙江嘉兴,314000
2. 武汉市华中科技大学同济医学院附属协和医院妇产科 |
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| 摘 要： | 目的:观察人滋养细胞(TEV-1)经血清剥夺刺激后,VEGF、Netrin-1的表达及分布,同时监测TEV-1增殖、凋亡及管腔形成等生物学行为的变化,探讨血清剥夺对滋养细胞生物行为的影响及其机制。方法:行早孕期滋养细胞株TEV-1培养,并随机分为实验组(无血清培养基处理)及对照组(15%胎牛血清培养基处理),应用MTT比色法、流式细胞术(FCM)检测细胞增殖凋亡情况,并监测管腔形成的变化。同时利用免疫荧光细胞化学染色法及实时定量PCR检测VEGF、Ne-trin-1蛋白表达定位及mRNA变化情况。结果:MTT及FCM检测表明,血清剥夺能抑制TEV-1细胞增殖、诱导TEV-1凋亡。滋养细胞培养24 h后可出现管腔形成现象,且这一现象不受血清剥夺的抑制。VEGF、Netrin-1蛋白均表达于TEV-1细胞胞浆,此外,实验组TEV-1VEGF mRNA与Netrin-1mRNA的表达水平依次为对照组的(0.94±0.02)、(0.62±0.11)倍。结论:血清剥夺后,滋养细胞出现凋亡增加及增殖抑制现象,并且VEGF、Netrin-1表达减少。血清剥夺可能通过影响VEGF、Netrin-1的表达来参与对滋养细胞增殖凋亡的调控。
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| 关 键 词： | 血清剥夺 滋养细胞 增殖 凋亡 |
Effects of serum deprivation on biological behaviors of trophoblast and the related mechanism |
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| ZHOU Xiao-Yu,LIU Xia,JIN Xiao-Ying et al.Effects of serum deprivation on biological behaviors of trophoblast and the related mechanism[J].Maternal and Child Health Care of China,2011,26(30):4752-4754. |
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| Authors: | ZHOU Xiao-Yu LIU Xia JIN Xiao-Ying |
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| Affiliation: | ZHOU Xiao-Yu,LIU Xia,JIN Xiao-Ying et al.Department of Obstetrics,Affiliated Maternal and Child Health Hospital of Jiaxing College,Jiaxing 314000,Zhejiang,China |
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| Abstract: | Objective:To observe the expressions and distributions of vascular endothelial growth factor(VEGF) and Netrin-1 after serum deprivation stimulation of human TEV-1 cells,meanwhile,monitor the changes of biological behaviors including proliferation,apoptosis and tubal formation of TEV-1,explore the effect of serum deprivation on biological behaviors of trophoblast and the related mechanism. Methods:TEV-1 cell line was cultured during the first trimester of pregnancy,then TEV-1 cells were divided into experime... |
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| Keywords: | Serum deprivation Trophoblast Proliferation Apoptosis |
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