Impact of β-glucan on the Fecal Water Genotoxicity of Polypectomized Patients |
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| Authors: | Katja T Turunen Vasiliki Pletsa Panagiotis Georgiadis John K Triantafillidis Dimitrios Karamanolis |
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| Affiliation: | 1. Laboratory of Biology, Biochemistry, Physiology and Microbiology, Harokopio University, 70, El Venizelou str., Kallithea, Greece;2. Institute of Biology, Medicinal Chemistry and Biotechnology, National Hellenic Research Foundation, Athens, Greece;3. IBD Unit, IASO General Hospital, Athens, Greece;4. Second Department of Gastroenterology, Evangelismos Hospital, Athens, Greece |
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| Abstract: | The aim of the study was to determine the effect of β-glucan on the cytotoxicity and genotoxicity of polypectomized patient's fecal water (FW). Polypectomized volunteers (n = 69) were randomly assigned to consume bread with or without β-glucan, for 3 months. FW was collected at the beginning (t = 0), the 30th and 90th day and 2 wk after the intervention. Cytotoxicity and genotoxicity were estimated on Caco-2 cells, using trypan blue exclusion test and comet assay, respectively. Gastrointestinal symptoms were recorded and subjects kept a 3-day food diary at baseline and after completion. Trypan blue exclusion test revealed cell survival of approximately 87% after incubation with FW. The FW samples showed 49% genotoxicity at the baseline. Genotoxicity in the intervention group decreased during the trial reaching statistical significance on the 90th day compared to control. An increase was noticed 2 wk after the trial, but it still remained significantly lower compared to control. Group-specific analysis for β-glucan also revealed significant decrease in the genotoxicity on the 90th day compared to baseline. β-glucan ingestion in polypectomized patients significantly decreased the genotoxicity of their FW. Our findings suggest that β-glucan consumption could possibly provide protection against colon cancer development. |
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