THANK作用下T淋巴细胞的功能及表型分析

目的 观察THANK对T淋巴细胞的免疫调节功能。方法 在克隆和表达THANK基因的基础上,将THANK蛋白作用于B、T淋巴细胞,用^3H-TdR掺入法检测其对B、T淋巴细胞的共刺激作用,并用流式细胞仪观察THANK对T细胞的功能调节。结果 THANK除可共刺激B细胞的生长外;还可增强抗CD3单抗所引起的T细胞增殖,使外周成熟T细胞向效应功能转化,并可诱导活化后的T细胞,尤其是CD4^ T细胞,凋亡及其表型变化。结论 THANK可调节T细胞表型及功能变化。

The functional and phenotypic analysis of T lymphocytes under stimulation of THANK

Objective To study the function of THANK in T lymphocyte immune regulation. Methods On the basis of THANK gene cloning and expression, isolated T and B lymphocytes were stimulated by purified THANK protein and its function in immune regulation was investigated by 3HTdR incorperation and flow cytometric analysis. Results In addition to the costimulation on B cell growth, THANK promoted CD3 mediated T cells proliferation, activated resting peripheral blood T lymphocytes and induced apoptosis and the phenotypic change of activated T lymphocytes. Conclusion THANK is involved in the modulation of T cells phenotype and function.