基于电项针对失眠大鼠下丘脑免疫细胞因子影响的实验研究

目的 观察不同频率电项针对睡眠剥夺大鼠下丘脑内免疫因子白细胞介素-1β(IL-1β)、白细胞介素-6(IL-6)和肿瘤坏死因子-α(TNF-α)含量的影响,明确电项针对失眠的治疗作用,并探讨其发挥促眠效应的可能作用机制.方法 将50只健康雄性Wistar大鼠随机分成空白组、模型组、2 Hz刺激组、50 Hz刺激组、100 Hz刺激组,每组10只.采用对氯苯丙氨酸(PCPA)化法制作失眠大鼠模型.造模成功后,各频率刺激组连续1周进行电项针干预治疗,1次/日,30 min/次,空白组和模型组每日在相应时间仿照刺激组固定躯体,不进行任何处置.各组于治疗结束后处死大鼠后迅速分离下丘脑组织,运用酶联免疫吸附法(ELISA)测定IL-1β、IL-6和TNF-α含量.结果 模型组大鼠下丘脑中IL-1β、IL-6和TNF-α浓度明显低于空白组,差异具有统计学意义(P<0.05).与模型组比较,各频率刺激组IL-1β、IL-6和TNF-α浓度均升高,差异具有统计学意义(P<0.05);各频率刺激组之间比较,2 Hz刺激组下丘脑IL-1β、IL-6和TNF-α 含量高于50 Hz和100 Hz组(P<0.05),50Hz组和100Hz组间比较无统计学差异(P>0.05).结论 不同频率电项针治疗失眠均有效,各频率组电项针治疗比较,2Hz刺激组的促眠作用最好,其促眠作用的机制可能与调节下丘脑内免疫因子IL-1β、IL-6和TNF-α的含量相关.

Experimental Study of the Influence of Electro-nape-acupuncture on the Hypothalamic Immunogical Cytokines in Insomnia Rats

Objective To observe the influence of different frequency electro-nape-acupuncture on the hypothalamic im-munogical cytokines interleukin-1β (IL-1β), interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α)contents in rats with sleep deprivation, clear the function of electro-nape-acupuncture treatment for insomnia and discuss the possible mechanism of promoting sleep. Methods Fifty healthy male Wistar rats were randomly divided into normal group, model group, 2 Hz stimulation group, 50 Hz stimulation group, 100 Hz stimulation group, 10 rats in each group. The models of insomnia were established by using para-chlorophenylalanine (PCPA). After duplicating the models successfully, the rats of each different frequency treatment group were given electro-nape-acupuncture stimulation for one week, one time/day, 30 min/time. The normal and model groups were fixed in the corresponding time every day without any disposal. The rats in each group were sacrificed and the hypothalami were taken after treatment.The contents of IL-1β, IL-6 and TNF-α in hypothalamus were measured by enzyme-linked immunosorbent assay (ELISA) in each group. Results Hypothalamic IL-1β, IL-6 and TNF-α contents in the model group were significantly lower than those in the normal group (P<0.05). Compared with the model group, the IL-1β, IL-6 and TNF-α contents increased significantly in each different frequency treatment group (P<0.05). The hypothalamic IL-1β, IL-6 and TNF-α contents in 2 Hz stimulation group were higher than those in the 50 Hz and 100 Hz groups (P<0.05) and there were no statistically significant differences between the 50 Hz and 100 Hz groups (P>0.05). Conclusion Different frequency electro-nape-acupuncture all could effectively cure insomnia and the effect of 2 Hz group in promoting sleep is the best. The mechanism of promoting sleep may be related to regulating hypothalamic IL-1β, IL-6 and TNF-α contents.