羟基红花黄色素A对雌激素效应相关蛋白表达的影响

目的 探讨羟基红花黄色素A(hydroxysafflor yellow A,HSYA)对雌激素效应相关蛋白表达的影响.方法 培养雌激素受体阳性细胞T47D和特异性雌激素受体(estrogen receptor,ER)阴性细胞SK-BR-3,将1×10-7mol/L-1×10-9mol/L三种梯度浓度的HSYA作用于两种细胞,通过蛋白质免疫印迹法检测雌激素受体ERα 及ERβ、细胞外调解蛋白激酶1/2(ERK1/2)及p-ERK1/2、孕激素受体(progesterone receptor,PR)、抑癌基因P27等蛋白的表达情况.结果 与空白对照组相比,在T47D细胞中,高浓度的HSYA促进了ERα、ERβ、ERK1/2、p-ERK1/2、PR的表达,抑制了P27的表达(P<0.01);在SK-BR-3细胞中,HSYA显著抑制了ERK1/2的表达,但对p-ERK1/2的表达影响不明显(P>0.05).结论 HSYA在细胞内影响雌激素效应相关蛋白的表达情况不同,可能是通过调节ER表达及激活ERK信号通路而发挥雌激素样作用.

Influence of Hydroxysafflor Yellow A on Related Protein Expression of Estrogenic Effects

Objective To explore the effect of hydroxylsafflor yellow A (HSYA) on related protein of estrogenic effect. Methods The positive cells T47D and ER, and negative cells SK-BR-3 of estrogen receptor (ER) were cultured, and 1×10-7 mol/L× 10-9 mol/L of HSYA act on this two cells., negative cells SK-BR-3. The expression of protein ERα, ERβ, extracellular regu-lated protein kinases 1/2 (ERK1/2), p-ERK1/2, Progesterone Receptor (PR), P27 was detected by Western Blot. Results Com-pared with the blank control group, the high concentration HSYA promoted the expression of Erα, ERβ, ERK1/2, p-ERK1/2, PR, while inhibite the expression of P27 in T47D cells (P<0.01). HSYA showed significant inhibitory effects on ERK1/2, while had no obvious effects on p-ERK1/2 in SK-BR-3 cells (P>0.05). Conclusion HSYA has different influnces on related protein of estrogenic effect in the cells, which possiblly through regulating the expression of ER and activating the ERK sig-naling pathway.