| 通心络对异丙肾上腺素诱导的H9c2心肌细胞肥大及Cx43mRNA表达的影响 |
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| 引用本文： | 杨军,丁赛良,邓彪,王光辉,张勇,王苏燕,邝孛. 通心络对异丙肾上腺素诱导的H9c2心肌细胞肥大及Cx43mRNA表达的影响[J]. 中国实验方剂学杂志, 2012, 18(23): 191-194 |
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| 作者姓名： | 杨军 丁赛良 邓彪 王光辉 张勇 王苏燕 邝孛 |
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| 作者单位： | 南华大学附属第一医院心内科,湖南衡阳,421001 |
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| 基金项目： | 湖南省科技厅重点课题项目(2009SK2010);湖南省自然科学基金重点项目(11JJ2046) |
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| 摘 要： | 目的:观察通心络(Tongxinluo,TXL)超细干粉水提物对异丙肾上腺素(isoproterenol,Iso)诱导的H9c2心肌细胞肥大的作用,并探讨其对缝隙连接蛋白43(connexin 43,Cx43)mRNA表达的影响。方法:将培养的H9c2心肌细胞随机分为对照组、TXL组、Iso组和Iso+TXL组,通心络以通心络超细干粉水提物的形式加入,使用异丙肾上腺素诱导72 h后,采用相差显微镜测量细胞大小,BCA法测定蛋白浓度,运用逆转录聚合酶链式反应(RT-PCR)测定Cx43mRNA的表达。结果:Iso刺激H9c2心肌细胞72 h后,Iso组细胞直径增大,高于对照组(P<0.05);通心络对正常H9c2心肌细胞直径无明显影响,但能抑制Iso诱导的细胞直径增大(P<0.05)。Iso组蛋白浓度明显升高,高于对照组(P<0.05);通心络对正常H9c2心肌细胞蛋白浓度无明显影响,但能抑制Iso诱导的蛋白浓度的升高(P<0.05)。Iso组Cx43mRNA表达明显减少,低于对照组(P<0.05);通心络对正常H9c2心肌细胞Cx43mRNA表达无影响,但能够抑制Iso诱导的Cx43 mRNA表达的下调(P<0.05)。结论:通心络可以抑制Iso诱导的H9c2心肌细胞肥大及Cx43mRNA表达的下调。
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| 关 键 词： | 通心络 心肌肥大 H9c2心肌细胞 连接蛋白43 异丙肾上腺素 |
| 收稿时间： | 2012-04-16 |
Effect of Tongxinluo on H9c2 Cardiomyocytes Hypertrophy and Expression of Connexin43 mRNA Induced by Isoproterenol |
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| YANG Jun,DING Sai-liang,DENG Biao,WANG Guang-hui,ZHANG Yong,WANG Su-yan and KUANG Bei. Effect of Tongxinluo on H9c2 Cardiomyocytes Hypertrophy and Expression of Connexin43 mRNA Induced by Isoproterenol[J]. China Journal of Experimental Traditional Medical Formulae, 2012, 18(23): 191-194 |
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| Authors: | YANG Jun DING Sai-liang DENG Biao WANG Guang-hui ZHANG Yong WANG Su-yan KUANG Bei |
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| Affiliation: | Department of Cardiology, First Affiliated Hospital of Nanhua University, Hengyang 421001, China;Department of Cardiology, First Affiliated Hospital of Nanhua University, Hengyang 421001, China;Department of Cardiology, First Affiliated Hospital of Nanhua University, Hengyang 421001, China;Department of Cardiology, First Affiliated Hospital of Nanhua University, Hengyang 421001, China;Department of Cardiology, First Affiliated Hospital of Nanhua University, Hengyang 421001, China;Department of Cardiology, First Affiliated Hospital of Nanhua University, Hengyang 421001, China;Department of Cardiology, First Affiliated Hospital of Nanhua University, Hengyang 421001, China |
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| Abstract: | |
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| Keywords: | Tongxinluo cardiac hypertrophy H9c2 cardiomyocytes connexin 43 isoproterenol |
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