Whole genome scan detects an allelic variant of <Emphasis Type="Italic">fad2</Emphasis> associated with increased oleic acid levels in maize |
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Authors: | André Beló Peizhong Zheng Stanley Luck Bo Shen David J Meyer Bailin Li Scott Tingey Antoni Rafalski |
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Affiliation: | (1) Graduate Integrative Training Option program, Department of Plant and Soil Sciences, University of Delaware, 152 Townsend Hall, Newark, DE 19716, USA;(2) Pioneer Hi-Bred International, Johnston, IA 50131-0184, USA;(3) DuPont Crop Genetics Research, DuPont Experimental Station E353, Route 141 and Henry Clay Road, Wilmington, DE 19880-0353, USA |
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Abstract: | We used whole genome scan association mapping to identify loci with major effect on oleic acid content in maize kernels. Single
nucleotide polymorphism haplotypes at 8,590 loci were tested for association with oleic acid content in 553 maize inbreds.
A single locus with major effect on oleic acid was mapped between 380 and 384 cM in the IBM2 neighbors genetic map on chromosome
4 and confirmed in a biparental population. A fatty acid desaturase, fad2, identified ∼2 kb from the associated genetic marker, is the most likely candidate gene responsible for the differences in
the phenotype. The fad2 alleles with high- and low-oleic acid content were sequenced and allelic differences in fad2 RNA level in developing embryos was investigated. We propose that a non-conservative amino acid polymorphism near the active
site of fad2 contributes to the effect on oleic acid content. This is the first report of the use of a high resolution whole genome scan
association mapping where a putative gene responsible for a quantitative trait was identified in plants.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users.
Nucleotide sequence data reported are available in the DDBJ/EMBL/GenBank databases under the accession number EF687907. |
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Keywords: | Whole genome scan Association mapping Maize fad2 Oleic fatty acid |
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