宫颈鳞癌组织中细胞凋亡的表达与临床生物学行为的关系

目的 :探讨细胞凋亡在宫颈鳞癌组织中的表达及其与宫颈鳞癌组织学分级、盆腔淋巴结转移及临床分期的关系。方法 :采用DNA末端缺口标记技术和免疫组化技术 ,检测 15例正常宫颈组织 ,2 2例CINⅠ /Ⅱ ,16例CINⅢ和 5 2例浸润性宫颈鳞癌 (ISCC)组织中细胞凋亡标记指数 (A LI)、增殖细胞核抗原标记指数 (PCNA LI) ,并分析A LI与宫颈鳞癌组织学分级、盆腔淋巴结转移及临床分期的关系。结果 :在正常宫颈上皮未检测到凋亡细胞 ,随着CIN病变的进展 ,A LI增高 (P <0 0 5 ) ,但在ISCC ,A LI明显低于CIN(P <0 0 1) ;PCNA LI随宫颈鳞癌各级病变的进展逐渐增高 (P <0 0 5 ) ;A LI随宫颈鳞癌组织分化级别的增高而减少 (P <0 0 5 ) ,在临床Ⅱ期组低于I期组 (P <0 0 5 ) ,盆腔淋巴结转移组低于无转移组 (P <0 0 5 )。结论 :细胞凋亡在宫颈鳞癌发生发展过程中以抑制细胞增殖而发挥负调控作用 ,在ISCC阶段调控紊乱。细胞凋亡与宫颈鳞癌的临床生物学行为相关。检测细胞凋亡有助于宫颈鳞癌进展及预后的评估

Relationship between apoptosis and clinical biological behavior of squamous cervical carcinoma

Aim：To analysis the relationship between apoptosis and the clinical biological behavior of squamous cervical carcinoma．Methods： The apoptotic labeling indices(A-LI) and PCNA(proliferating cell nuclear antigen)-LI， were observed in 15 cases of normel cervical epithelia, 22 cases of CINⅠ/Ⅱ,16 cases of CINⅢ and 52 cases of ISCC (invasive squamous cervical carcinoma) tissues by using DNA nick end labeling technique and immunohistochemical staining.Results：No apoptotic cell was detected in normal cervical epithelium, A-LI increased with CINⅠ/Ⅱ→CINⅢ(P<0.05), A-LI in ISCC were significantly lower than those in CIN(P<0.01).PCNA-LI gradually increased with the development of every lesion grade of cervical carcinomas (P<0.05). A-LI decreased with the clinical stage and the differentiation degree progression, and were lower in pelvic lymphatic metastasis group than that without lymphatic metastasis. Conclusion: Apoptosis may play a negative regulating action by inhibiting excessive proliferation of cell during the developing course of squamous cervical carcinoma, and this regulating was inordinate in ISCC, Apoptosis is correlated to clinical biological behavior. The detection of apoptosis could contribute to evaluate the progress and prognosis of squamous cervical carcinoma.