自然排卵与注射人绒毛膜促性腺激素促排卵时阴道脱落细胞雌、孕激素受体的研究

目的：研究排卵期阴道脱落细胞的雌、孕激素受体。方法：自然排卵妇女、注射人绒毛膜促性腺激素（ｈｕｍａｎ ｃｈｏｒｉｏｎｉｃ ｇｏｎａｄｏｔｒｏｐｉｎ，ｈＣＧ）促排卵妇女排卵期阴道脱落细胞，应用免疫组织化学ＡＢＣ 法和图像分析技术进行雌激素受体（ｅｓｔｒｏｇｅｎｒｅｃｅｐｔｏｒ，ＥＲ） 和孕激素受体（ｐｒｏｇｅｓｔｅｒｏｎｅｒｅｃｅｐｔｏｒ，ＰＲ）的定位和定量研究。结果：两组组内排卵前、后阴道脱落细胞ＥＲ比较均无差异；注射ｈＣＧ 后阴道脱落细胞的ＥＲ在排卵前和排卵后均较自然排卵组有明显增高；自然排卵组ＰＲ在排卵后较之排卵前明显升高。结论：排卵前后阴道上皮细胞ＥＲ 无明显变化；ｈＣＧ可提高阴道上皮细胞ＥＲ 水平；排卵后阴道上皮细胞ＰＲ增加。

Study of estrogen receptors and progesterone receptors in epithelial cells of vagina in natural ovulation and ovulation by injection of human chorionic gonadotropin

Objective: To study the estrogen receptors(ER) and progesterone receptors(PR) in vagina epithelial cells during ovulation phase. Methods: Immunohistochemical ABC method and Q 500 image analysis system were used to locate and quantitative determinate of ER and PR in vagina epithelial cells in the nature ovulation women and injection of human chorionic gonadotropin (hCG) to induce ovulation women. Results: ER and PR positive immunostaining was found in the cytoplasm and nuclei of the vagina epithelial cells . ER level in vagina epithelial cells within the two groups had no difference in pre ovulation and post ovulation respectively; the ER level in the vagina epithelial cells in of hCG injection group was higher than that in natural ovulation group no matter which was in pre ovulation or post ovulation. The PR level in the vagina epithelial cells increased in post ovulation. Conclusion: hCG could increase the ER level in the vagina epithelial cells; After ovulation, the PR level in vagina epithelial cells increased.