| SCP对人乳腺癌细胞增殖抑制作用的研究 |
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| 引用本文： | 张敏,陈小义,陈晓,陈莉.SCP对人乳腺癌细胞增殖抑制作用的研究[J].武警医学院学报,2002,11(1):4-6,F003. |
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| 作者姓名： | 张敏 陈小义 陈晓 陈莉 |
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| 作者单位： | 1. 武警医学院,生物教研室,天津,300162
2. 武警医学院,成果转化处,天津,300162 |
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| 摘 要： | 目的：研究SCP对人乳腺癌细胞生长，抑制效应，探讨其抗癌作用机制。方法：用不同浓度的SCP加入体外培养的人乳腺癌细胞（MCF－7）中，观察加药后细胞生长和有丝分裂指数（MI）的变化，用MTT法检测其细胞毒作用；Hoechst33342/PI荧光双染色方法检测细胞凋亡。结果：SCP明显抑制MCF－7细胞生长，IC50值为1mg/ml;MI于加药后48h较对照组明显降低；凋亡细胞比例在用药后48h达63．3％，结论：SCP可有效抑制人乳腺癌细胞的增殖。
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| 关 键 词： | 人乳腺癌细胞 鲨鱼软骨制剂 细胞增殖 抗癌机制 体外实验 |
| 文章编号： | 1008-5041(2002)01-0004-03 |
Effect of shark cartilage preparation on proliferation of MCF-7 cells |
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| ZHANG Min,CHEN Xiao yi,CHEN Xiao,CHEN Li.Effect of shark cartilage preparation on proliferation of MCF-7 cells[J].Acta Academiae Medicinae CPAPF,2002,11(1):4-6,F003. |
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| Authors: | ZHANG Min CHEN Xiao yi CHEN Xiao CHEN Li |
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| Affiliation: | ZHANG Min,CHEN Xiao yi,CHEN Xiao,CHEN Li . Department of Cell Biology,Medical College of Chinese People's Armed Police Forces,Tianjin 300162 |
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| Abstract: | Objective:To study the effects and its mechanism of shark cartilage preparation on human breast cancer cells line proliferation. Methods: Using experiment in vitro, we added SCP of different concentrations into the medium in which the cells were cultured. The changes of cell proliferation were assessed by MI. Cytoxicity rate was detected by MTT. The number of apoptotic cells was quantitatively assessed by fluorescent light microscope. Results: The cell growth was remarkably inhibited by SCP; the IC50 values of SCP for MCF-7 cells was 1mg/ml;after 48h of exposure to SCP, MI was remarkably reduced.,the rate of Apoptotic cells was increased. Conclusion:Malignant phenotype of MCF-7 was changed and its proliferation ability was remarkably inhibited by SCP. < |
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| Keywords: | Human breast cancer cell line Shark cartilage preparation Cell proliferation |
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