针刺对胚胎着床障碍大鼠黄体功能的影响

目的:观察针刺对胚胎着床障碍大鼠黄体功能的影响,探讨其作用机制.方法:将早孕大鼠随机分为正常组(N)、模型组(M)、针刺穴位组(A)、针刺非穴位组(AC),M组、A组、AC组均采用米非司酮造模.A组针刺大鼠双侧"后三里""三阴交",AC组针刺其穴位旁开非穴位点.放免法检测各组大鼠血清黄体生成素(LH)、雌二醇(E_2)、孕酮(P)的水平;免疫组化、Western-blot方法检测各组大鼠卵巢组织血管内皮生长因子(VEGF)的表达;RT-PCR方法检测各组卵巢组织黄体生成素受体(LHR)mRNA及VEGF mRNA的表达.结果:A组血清LH、P水平显著高于M组、AC组(均P<0.05),与N组比较差异无统计学意义;A组卵巢组织VEGF含量、LHR mRNA、VEGF mRNA表达水平较M组、AC组显著提高(均P<0.05),与N组比较差异无统计学意义.结论:针刺"后三里""三阴交"可升高胚胎着床障碍大鼠血清P、LH水平,上调卵巢组织LHR mRNA、VEGF及其mRNA表达水平,可在一定程度上增强大鼠黄体功能,改善胚胎着床环境.

Effects of acupuncture on the luteal function of rats with dysfunctional embryo implantation

Objective To observe the influence of acupuncture on the luteal function of rats with dysfunctional embryo implantation,and investigate its mechanism. Methods The early pregnant female rats were randomly divided into a normal control group,a model group,an acupoint group and a non-acupoint group.The model rats with dysfunctional embryo implantation were established with Mifepristone.For acupuncture treatment,bilateral"Housanli"(ST 36)and"Sanyinjiao"(SP 6)were selected in the acupoint group,while the points around the acupoint were used in the non-acupoint group.In this experiment,the levels of luteinizing hormone(LH),estrodio](E_2)and progesterone(P)in serum were tested with radioimmunity method,the expression of vascular endothelial growth factor(VEGF)in ovary was examined with immunohistochemical assay and Western-blot,and also,the mRNA expression of VEGF and luteinizing hormone receptor(LHR)in ovary were detected with RT-PCR. Results The levels of LH and P in serum,as well as the expression of VEGF,VEGF mRNA and LHR mRNA in ovary in the acupoint group were significantly higher than those in the model group and the non-acupoint group(P<0.05),but there was no obvious difference from that of the normal control group. Conclusion Acupuncturing at"Housanli"(ST 36)and"Sanyinjiao"(SP 6)can increase the levels of LH and P in the serum,and up-regulate the expression of VEGF,VEGF mRNA and LHR mRNA in ovary,which may enhance the luteal function of rats with dysfunctional embryo implantation and improve its embryo implantation.