甘氨酸对缺氧/复氧心肌细胞[[Ca2+]i和TNF-α浓度的影响

目的: 观察甘氨酸对缺氧/复氧心肌细胞内游离钙、心肌细胞存活率和TNF-α浓度的影响。方法: 利用原代培养的SD大鼠乳鼠心肌细胞建立心肌缺氧/复氧(H/R)模型,实验分7组:① 对照组;② 缺氧/复氧 (H/R)组;③ 0.5 mmol/L甘氨酸(GLY)+H/R组;④ 1.0 mmol/L GLY+H/R组;⑤ 2.0 mmol/L GLY+H/R组;⑥ 4.0 mmol/L GLY+H/R组;⑦ 4.0 mmol/L GLY对照组。结果: 在一定浓度范围内(0.5-2.0 mmol/L),甘氨酸能抑制缺氧/复氧损伤引起的细胞内钙超载,并呈现剂量依赖性关系,在2.0 mmol/L浓度水平,达到最佳抑制效应。甘氨酸能抑制心肌细胞产生TNF-α,避免心肌H/R损伤的加重,增加心肌细胞的存活率。 结论:甘氨酸对缺氧/复氧心肌具有保护作用,其机制与抑制钙超载,减少TNF-α的生成有关。

Effects of glycine on intracellular free calcium and tumor necrosis factor-α in cardiomyocytes during hypoxia/reoxygenation

AIM: To observe the influence of glycine on intracellular free calcium, the concentration of tumor necrosis factor-α and the survival rate of myocardial cells during hypoxia/reoxygenation (H/R). METHODS: The simulated model of myocardial ischemia-reperfusion with the primary cultured cardiomyocytes of neonatal rats was established, and the cultured cardiomyocytes were divided into seven groups, control group, hypoxia/reoxygenation group, glycine (0.5 mmol/L) plus hypoxia/reoxygenation group, glycine (1.0 mmol/L) plus hypoxia/reoxygenation group, glycine (2.0 mmol/L) plus hypoxia/reoxygenation group, glycine (4.0 mmol/L) plus hypoxia/reoxygenation group, 4.0 mmol/L glycine group. RESULTS: Within certain concentration (0.5-2.0 mmol/L), the glycine could inhibit the calcium overload resulting from hypoxia/reoxygenation injury in cells in a dose-dependent manner with the optimal inhibitory effect at 2.0 mmol/L. Glycine inhibited the secretion of tumor necrosis factor-α from myocardial cells and increased the survival rate of myocardial cells. CONCLUSION: Glycine has a protective effect on hypoxia/reoxygenation myocardial cells, which may be related to inhibiting calcium overload and decreasing the production of tumor necrosis factor-α.