Influence of glucose feeding on the ligninolytic enzyme production of the white-rot fungus Phanerochaete chrysosporium |
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Authors: | Zhou Xiaoyan Wen Xianghua and Feng Yan |
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Affiliation: | (1) The State Key Joint Laboratory of Environmental Simulation and Pollution Control, Department of Environmental Science and Engineering, Tsinghua University, Beijing, 100084, China |
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Abstract: | The present work studied the influence of glucose feeding on the ligninolytic enzyme production of Phanerochaete chrysosporium in a nitrogen-limited (C/N ratio is 56/8.8 mmol/L) medium. Several sets of shaking flask experiments were conducted. The
results showed that 2 g/L glucose feeding on the first day of the culture (24 h after the inoculation) simulated both fungal
biomass growth and enzyme production. The manganese peroxidase (MnP) activity was 2.5 times greater than that produced in
cultures without glucose feeding. Furthermore, the glucose feeding mode in fed-batch culture was also investigated. Compared
to cultures with glucose feeding every 48 h, cultures with glucose feeding of 1.5 g/L (final concentration) every 24 h produced
more enzymes. The peak and total yield of MnP activity were 2.7 and 3 times greater compared to the contrast culture, respectively,
and the enzyme was kept stable for 4 days with an activity of over 200 U/L.
Translated from Acta Scientise Circumstantiae, 2007, 27(3): 363–368 译自: 环境科学学报] |
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Keywords: | white-rot fungus ligninolytic enzymes glucose feeding fed-batch culture |
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