胃粘膜相关淋巴组织型淋巴瘤MAPK和Stat3磷酸化及cyclinD1蛋白表达的意义

目的:研究胃粘膜相关淋巴组织型(MALT)淋巴瘤MAPK和Stat3磷酸化与cyclinD1蛋白表达及其意义。方法:利用免疫组织化学方法检测了45例胃MALT淋巴瘤MAPK和Stat3磷酸化及cyclinD1蛋白的表达。结果:在胃MALT淋巴瘤中p-MAPK、p-Stat3及cyclinD1蛋白的阳性率分别为73.3%(33/45)、64.4%(29/45)和68.9%(31/45);低度恶性组p-MAPK和cyclinD1蛋白的阳性表达强度明显高于高度恶性组(P<0.01),而p-Stat3表达强度无明显差异(P>0.05);在低度和高度恶性胃MALT淋巴瘤中p-MAPK和cyclinD1蛋白的阳性信号强度均呈明显的正相关(r=0.6572和0.6823,P<0.01),而p-Stat3与cyclinD1蛋白表达未见明显相关性(r=0.1927,P>0.05)。结论:提示MAPK磷酸化在胃MALT淋巴瘤中发生及演进过程中起重要作用,但Stat3的磷酸化可能与该肿瘤的恶性演进关系不明显;在胃MALT淋巴瘤的发生与发展中,p-MAPK可诱导cyclinD1过度表达,从而促使该肿瘤细胞维持高增殖状态。

THE SIGNIFICANCE OF MAPK AND Stat3 PHOSPHORYLATION AND CyclinD1 PROTEIN EXPRESSION IN LYMPHOID TISSUE LYMPHOMA ASSOCIATED WITH GASTRIC MUCOSA

Objective:To investigate the significance of MAPK and State3phosphorylation and cyclinD1protein expression in gastric mucosa associated lymphoid tissue(MALT)lymphoma.Method:SP immunohistochemistry was used to detect expressions of p-MAPK,p-Stat3and cyclinD1protein in45cases of gastric MALT lymphoma HL.Results:Of 45MALT lymphomas,the positive rates of p-MAPK,p-Stat3and cyclinD1protein were73.3%(33/45),64.4%(29/45)and68.9%(31/45)respectively.The positive intensities of p-MAPK and cyclinD1protein in low grade group were significantly higher than in high grade group(P<0.01),whereas that of p-Stat3did not show significantly difference(P>0.05)in all cases.There was a positive relationship between positive signal intensities of p-MAPK and cyclinD1protein in low and high groups of gastric MALT lymphomas(r=0.6572and0.6823,P<0.01),but the correlation was not found between p-Stat3and cyclinD1protein expression(r=0.1927,P>0.05).Conclusion:The data suggest that activation of MAPK might play an important role in genesis and progression of gastric MALT lymphomas.MAPK may induce overexpression of cyclinD1protein and result in persistent prolif eration of these tumor cells in gemesis of gastric MALT lymphomas.