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| 发酵红皮萝卜中硝酸还原菌的分离筛选 | |
| 引用本文： | 郭双霜,葛焱,陈安均,蒲彪,曾骏,付莎莉.发酵红皮萝卜中硝酸还原菌的分离筛选[J].食品与发酵工业,2012,38(12). |
| 作者姓名： | 郭双霜 葛焱 陈安均 蒲彪 曾骏 付莎莉 |
| 作者单位： | 1. 四川农业大学食品学院,四川雅安,625014 2. 南京旅游职业学院酒店管理系,江苏南京,211100 3. 四川农业大学食品学院,四川雅安,625014;四川省农产品加工及贮藏工程重点实验室,四川雅安,625014 |
| 基金项目： | 国家自然科学基金资助项目 |
| 摘 要： | 研究了发酵红皮萝卜中细菌和大肠杆菌数量与亚硝酸盐含量变化的规律,从中分离得到10株菌株,通过硝酸盐还原能力的测定,筛选出9株具有硝酸盐还原能力的菌株,其中E5和E6两个菌株硝酸盐还原能力最强,并测定了2个菌株硝酸还原酶(NR)的酶活及pH对酶活的影响。进而对筛选的E5、E6两个菌株进行了分子生物学鉴定。结果表明,E5为阴沟肠杆菌(Enterobacter cloacae),E6为克雷伯氏菌(Klebsiella oxytoca)。 |
| 关 键 词： | 发酵红皮萝卜 阴沟肠杆菌 克雷伯氏菌 硝酸还原酶 |
Isolation Identification of Nitrate Reducing Bacteria from Fermented Radish |
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| Abstract: | The relationship between the number of bacteria,Escherichia coli and nitrite was studied.Total ten bacteria strains were isolated from fermented radish,wherein nine nitrrate reducing bacteria were screened by the determination of conversion ability of nitrate to nitrite.Among them,the strains E5 and E6 had the highest nitrate reducing activity.Furthermore,nitrate reductase activity of these two strains and the influence of pH on such activity were analysed.According to the biochemical and molecular identification,E5 was identified as Enterobacter cloacae and E6 was Klebsiella oxytoca. |
| Keywords: | fermented radish Enterobacter cloacae Klebsiella oxytoca nitrate reductase |
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