神经干细胞转基因疗法与运动性脊髓损伤的治疗

背景：神经干细胞基因疗法的提出,给运动性脊髓损伤的修复与治疗提供了广阔的应用前景和挑战。目的：综述神经干细胞的增殖分化和基因调控促进运动性脊髓损伤恢复的效果。方法：应用计算机检索Medline数据库、维普数据库和清华同方数据库1991-01/2010-12有关神经干细胞疗法治疗运动性脊髓损伤的文献,以＂neural stem cells,gene therapy,exercise spinal cord injury,gene modification,gene transfection＂为英文检索词,以＂神经干细胞,脊髓损伤,基因治疗＂为中文检索词,排除重复性、陈旧性研究,对所得文献资料进行整理,运用归纳演绎等方法进行分析。结果与结论：神经干细胞的基因治疗能够通过其神经营养因子的修饰、增殖分化和移植技术,以替代坏死、凋亡的神经细胞,并在损伤区域分泌大量的神经营养因子,达到改善局部微环境,促进神经通路的重建、新生神经元的增殖等促进运动性脊髓损伤的再生与修复。同时,神经干细胞基因治疗运动性脊髓损伤还面临着巨大的考验,包括神经干细胞基因治疗技术的成熟性、转基因后的稳定高效表达性等相关问题还没有得到很好的解决,仍需要不断的进行基础性研究和临床实验。

Neural stem cell gene therapy for exercise-induced spinal cord injury

BACKGROUND：The neural stem cell gene therapy proposed for sports rehabilitation and treatment of spinal cord injury provides a broad application prospects and challenges.OBJECTIVE：To review proliferation and differentiation of neural stem cells as well as neural stem cells gene therapy effect on exercise-induced spinal cord injury.METHODS：Medline,VIP,and CNKI databases（1991-01/2010-12） were retrieved for articles about neural stem cell gene therapy for exercise-induced spinal cord injury.The keywords were ＂neural stem cells,gene therapy,exercise spinal cord injury,gene modification,gene transfection＂ in English,and ＂neural stem cells,spinal cord injury,gene therapy＂ in Chinese.Repetitive and old papers were excluded,and the other papers were organized and analyzed using induction and deduction methods.RESULTS AND CONCLUSION：Through modification of neurotrophic factors,proliferation,differentiation and transplantation,the neural stem cell gene therapy can replace necrotic and apoptotic nerve cells in the damaged area and a large number of neurotrophic factors were secreted to improve the local micro-environment for the reconstruction of neural pathways,such as the proliferation of new neurons to promote exercise-induced spinal cord injury.Meanwhile,the neural stem cell gene therapy applied to exercise-induced spinal cord injury also faces with enormous challenges,including whether the neural stem cell gene therapy is mature,high-stable transgenic expression,and other relevant problems,which still need basic research and clinical trials to be addressed.