中药接骨Ⅰ号治疗超声透入桡骨骨折愈合过程中骨形态发生蛋白7的表达

背景：将低强度脉冲超声波与中药相结合,即中药超声透入疗法,在动物实验和临床中用于骨折的治疗并取得了确切的疗效,然而其促进骨折愈合机制的研究却少有报道。目的：观察中药超声透入对新西兰大白兔桡骨中段骨缺损愈合过程中骨形态发生蛋白7表达的影响。方法：84只兔构建双侧桡骨中段骨缺损的骨折模型,建模成功后随机分成3组：中药组给予以接骨Ⅰ号为耦合剂的低强度脉冲超声透入治疗,超声组每日于骨折部位予以普通超声耦合剂的低强度脉冲超声治疗,对照组行假刺激。分别于术后第3,6,9,12,15天取骨痂行采用RT-PCR和免疫组织化学染色法检测骨形态发生7mRNA表达和平均吸光度值变化,第4和6周行X射线片检查以观察不同治疗方法对骨缺损愈合的疗效。结果与结论：X射线检测结果显示中药组骨折愈合效果最好。中药组骨形态发生蛋白7mRNA表达和平均吸光度值均高于超声组和对照组（P〈0.05）,在术后第9和12天最较明显（P〈0.01）。结果证实,中药低强度脉冲超声透入疗法能促进兔桡骨骨折的修复、缩短骨折修复时间,其修复作用与促进骨形态发生蛋白7表达有关。

Effect of herbal ultrasound-phoresis on bone morphogenetic protein 7 expression during radialis fracture healing

BACKGROUND：Some scholars combined low-intensity pulsed ultrasound（LIPUS） with herbal（herbal ultrasound-phoresis） and this therapy had a good curative effect while being used for treating fractures in animal experiment and clinical treatment.However,there is fewer report about the mechanism that herbal ultrasound-phoresis promotes fracture healing.OBJECTIVE：To investigate the effect on bone morphogenetic protein 7（BMP-7） expression during fracture healing treated by herbal ultrasound-phoresis in rabbits.METHODS：Animal models of radius fracture in rabbits were established.Totally 84 rabbits of New Zealand were divided into 3 groups after successful model preparation.The experimental group were treated by herbal ultrasound-phoresis,the ultrasound group were treated by LIPUS,the control group were given sham exposure.The rabbits were sacrificed at 3,6,9,12,15 days and 4,6 weeks respectively after operation.The changes of BMP-7 mRNA expression and mean absorbance value were detected by RT-PCR and immunohistochemical examinations;the fracture healing was assessed through X-ray at 4 and 6 weeks after operation.RESULTS AND CONCLUSION：X-ray showed that the fracture in the experimental group was healed faster than the ultrasound group and the control group.The expressions of BMP-7 mRNA and mean absorbance value in the experimental group were higher than that in ultrasound group and the control group（P〈0.05）,which was particularly obvious at 9,12 days（P〈0.01）.Herbal ultrasound-phoresis might accelerate fracture healing by increasing the expression of BMP-7.