| 带活性末端的热敏高分子的制备及其在荧光免疫分析中的应用 |
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| 引用本文： | 杨黄浩,朱庆枝,李东辉,丁马太,许金钩.带活性末端的热敏高分子的制备及其在荧光免疫分析中的应用[J].高等学校化学学报,2001,22(5):754-758. |
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| 作者姓名： | 杨黄浩 朱庆枝 李东辉 丁马太 许金钩 |
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| 作者单位： | 1. 厦门大学化学化工学院现代分析教育部重点实验室;
2. 厦门大学抗癌研究中心, 厦门大学化学系, 厦门 361005 |
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| 基金项目： | 教育部博士点基金! (批准号 :970 3 840 7，福建省自然科学基金! (批准号 :B9810 0 0 4)资助 |
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| 摘 要： | 合成了带有活性末端的寡聚N-异丙基丙烯酰胺(ONIPAAm),并考察了其温度敏感性质.以ONI-PAAm作为免疫分析的载体与鼠IgG偶联,以四磺基铁酞菁为标记物,以羊抗鼠IgG抗体为分析模型,建立了竞争型热敏相分离荧光免疫分析新系统.羊抗鼠IgG抗体在0～1500ng/mL范围内与体系相对荧光强度呈良好的线性关系,检测限为2ng/mL.
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| 关 键 词： | 寡聚N-异丙基丙烯酰胺 温度敏感高聚物 热敏相分离 模拟酶 荧光免疫分析 |
| 文章编号： | 0251-0790（2001）05-0754-05 |
| 收稿时间： | 2000-06-09 |
Preparation of Temperature-sensitive Polymer with Reactive End Carboxylic Group and Its Application in the Fluoroimmunoassay |
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| YANG Huang-hao,ZHU Qing-Zhi,LI Dong-Hui,DING Ma-tai,XU Jin-guo.Preparation of Temperature-sensitive Polymer with Reactive End Carboxylic Group and Its Application in the Fluoroimmunoassay[J].Chemical Research In Chinese Universities,2001,22(5):754-758. |
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| Authors: | YANG Huang-hao ZHU Qing-Zhi LI Dong-Hui DING Ma-tai XU Jin-guo |
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| Affiliation: | Department of Chemistry, the Key Laboratory of Analytical Sciences of MOE, Cancer Rechearch Center, Xiamen University, Xiamen 361005, China |
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| Abstract: | A temperature sensitive polymer containing a terminal carboxyl group was prepared by copolymerization of N isopropylacrylamide with mercaptoacetic acid. This polymer was conjugated to the IgG with EDCI and used to develop a novel polymer mimetic enzyme competitive immunoassay method for determination of anti IgG with iron tetrasulfonatophthalocyanine as a labelling reagent to catalyze the reaction of p hydroxyphenyl acetic acid (HPA) and hydrogen peroxide in alkaline medium. The calibration graph for anti IgG was linear over the range of 0\_1 500 ng/mL with a detection limit of 2.0 ng/mL. |
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| Keywords: | Oligo N isopropylacrylamide Temperature sensitive polymer Heat sensitive phase separation Mimetic enzyme Fluoroimmunoassay |
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