方斑东风螺2个养殖群体遗传变异的RAPD分析

运用RAPD技术对方斑东风螺(Babylonia areolata Lamarck)泰国养殖群体和海南养殖群体的遗传多样性进行研究。选取21个10 bp随机引物对方斑东风螺2个群体各20个个体进行RAPD分析,21个引物共检测出222条带。泰国养殖群体的多态位点比例为70.27%,Shannon多样性信息指数为0.1858,Nei’s基因多样性指数为0.249 1,群体内个体间平均遗传相似率为0.7920,平均遗传距离为0.2080;海南养殖群体的多态位点比例为73.87%,Shannon信息指数为0.2044,Nei’s基因多样性指数为0.2615,群体内个体间平均遗传相似率为0.7620,平均遗传距离为0.2380。群体内遗传变异占种内遗传变异的76.81%,群体间遗传变异占23.19%。以上分析表明,方斑东风螺泰国养殖群体和海南养殖群体的遗传多样性水平仍较高,但海南群体的遗传多样性水平比泰国群体要高。

Genetic diversity of two cultured populations of Babylonia areolata revealed by RAPD analysis

Random amplified polymorphic DNA(RAPD) technique was applied to assess the genetic diversity of two cultured populations of B.areolata from Thailand and Hainan of China,respectively.Twenty individuals from each population were randomly selected.Twenty-one random primers were used and 222 bands were generated.The results showed that in the Thailand population the percentage of polymorphic fragments was 70.27;Shannon's index of phenotypic diversity was 0.1858;Nei's index of gene diversity was 0.2491;the mean genetic similarity was 0.7920,and the mean genetic distance was 0.2080.In Hainan population the percentage of polymorphic fragments was 73.87%;Shannon's index of phenotypic diversity was 0.2044;Nei's index of gene diversity was 0.2615;the mean genetic similarity was 0.7620,and the mean genetic distance was 0.2380.The percentage of genetic variations which came from the inside of the population was 76.81%,and that came from the outside of the population was 23.19%.Based on the results,the genetic diversity of the two populations of B.areolata from Thailand and Hainan were in good condition.However,the level of genetic diversity of Hainan population was higher than that of Thailand population