肥大细胞在单纯和放射复合伤口愈合中的作用

目的:观察肥大细胞(MC)及其亚群在单纯和放射复合伤口中的动态变化,旨在探讨MC在其中的作用及其机制。方法:应用阿里新兰－番红花红(ABS)双染色法,检测MC及其亚群。结果:①伤后2d两组MC总数均出现一致降低,伤后5-7dMC总数迅速升高并逐渐达到峰值,且一直维持到伤后15-28d。②粘膜型MC(MMC)和结缔组织型MC(CTMC)亚群也出现规律性变化。伤后2d,MMC和混合型MC(MixMC)均出现明显降低,此后一直维持在较低水平。而CTMC伤后一直维持在较高水平。③伤照组MixMC在伤后5d、MC总数在伤后5-15d,均明显少于单伤组。结论:MC及其亚群在创伤愈合以及辐射延迟伤口愈合过程中起着重要作用。

Role of mast cell and its subpopulations in simple and irradiated-wound of rats

AIM:To examine the role and dynamic changes of mast cell(MC) and its subpopulations in simple and irradiated-wound of rats.METHODS:MC and its subpopulations were estimated using alcian blue-safranin (ABS)double staining RESULTS:(1)The total number of MC in two groups decreased coincidently on day 2 after wounding, and the total MC increased rapidly and reached maximal gradually on day 5 and 7 after wounding, the increment of MC remained consistently 28 day after wounding (2)Both mucosal MC( MMC) and Mix MC decreased obviously on day 2 after wounding, hereafter,they remained the low level all the time However, the CTMC kept in the high level after wounding (3)The Mix MC on day 5 and the total MC during day 5-15 after wounding were lower in irradiated group than in simple wound group CONCLUSION: MC and its subpopulations could delay the healing process of simple and irradiated wound