MMP-2 mRNA及蛋白的表达在原发性 肝细胞癌组织中的意义

 目的 探讨原发性肝细胞癌（HCC）组织中基质金属蛋白酶-2（MMP-2）mRNA和MMP-2蛋白的表达,及其与临床病理参数之间的关系。方法 应用RT-PCR技术、免疫组织化学Maxvison^TM法，分别检测47例HCC组织及其癌旁组织、20例正常肝组织中MMP-2 mRNA和MMP-2蛋白的表达情况。结果（1）MMP-2 mRNA和MMP-2蛋白在肝癌中的表达分别为70.2%（33/47）和63.8％（30/47），均高于癌旁组织12.7％（6/47）和正常组织10％（2/20）, 差异有统计学意义(P＜0.01), 癌旁组织和正常组织中MMP-2的表达差异无统计学意义(P>0.05)；MMP-2mRNA和MMP2蛋白在肝癌中表达的两种检测方法差异无统计学意义(P>0.05)；（2）癌组织中MMP-2的表达在TNM分期Ⅲ～Ⅳ期组中的阳性表达率高于Ⅰ～Ⅱ期组(P＜0.05)，血管内有肿瘤栓组MMP 2阳性率明显高于无瘤栓组(P＜0.05），有转移组的阳性率高于无转移组(P＜0.05）。结论 MMP-2的表达与HCC的TNM分期、血管内有无瘤栓和转移密切相关，可作为判断HCC侵袭性的评估指标之一。

Expression and Significance of Matrix Metalloproteinase-2 mRNA and Protein in Primary Hepatocellular Carcinoma

Objective To investigate the expression of matrix metalloproteinase-2 (MMP-2) at mRNA and protein levels in primary hepatocellular carcinoma (HCC) and their relationship with clinicopathological parameters. Methods RT-PCR technique and Maxvision^TM immunohistochemical method were used to detect MMP-2 mRNA and protein levels in 47 paired HCC and cancer adjacent tissue samples and 20 normal liver tissues samples,respectively. Results The positive rates of expressions of MMP-2 mRNA and protein in 47 HCC cases were 70.2%（33/47）and 63.8％（30/47）, respectively,which were significantly higher than those in cancer adjacent tissues and in normal liver tissues (12.7％and 10.0％, respectively). The positive rates of MMP-2 expression in HCC were higher than that in adjacent non HCC liver tissue and in normal liver tissue and the difference was statistically significant (P＜0.01);the difference of MMP-2 expressions between adjacent non HCC liver tissue and normal liver tissue had no statistically significance (P>0.05). The two methods had no statistically significance (P>0.05).② The high positive rates of MMP-2 in TNM stages of Ⅲ~Ⅳ were significantly higher than those in stages of Ⅰ~Ⅱ(P＜0.05), and the positive rates of MMP-2 in HCC with metastasis was higher than that without metastasis(P＜0.05).The positive rate of MMP-2 in HCC with tumor thrombus was higher than that without thrombus (P＜0.05). Conclusion The expressions of MMP-2 are closely correlated with TNM staging, tumor thrombus and metastasis in HCC which may serve as predictors for determining tumor aggressive behavior in HCC.