非小细胞肺癌多药耐药因子的表达相关性及临床意义

探讨多药耐药因子在肺癌中表达与共表达的相关性及其临床意义。方法:采用免疫组化SP技术检测52例肺癌患者病理组织中P-糖蛋白（P-gp）、多药耐药相关蛋白(MRP)、谷胱甘肽S转移酶Ⅱ(GST-Ⅱ)3种多药耐药因子的表达水平。结果: 肺癌组织中P-gp、 MRP、GST-Ⅱ阳性表达率分别为51.9% (27/52)、61.5% (32/52)和78.8%（41/52）；耐药因子在不同病理类型、不同TNM分期和不同分化程度间表达差异无显著性(P＞0.05)；P-gp和MRP均阳性40.1%，P-gp和GST-Ⅱ均阳性44.2%，MRP和GST-Ⅱ均阳性50.0%，P-gp、MRP和GST-Ⅱ均阳性21.2%，其中P-gp与MRP间阳性表达率呈正相关关系(r_s=0.632,P<0.01），P-gp与GST-Ⅱ间阳性表达率呈正相关关系 (r_s=0.521,P<0.01），MRP与GST-Ⅱ间阳性表达率呈正相关关系(r_s=0.532, P<0.01）。结论: 肺癌的多药耐药现象是由多个耐药因子共同参与作用的结果，肺癌多药耐药的发生与不同的病理组织类型、不同TNM分期、不同分化程度间无关联

Coexpression and clinical significance of multidrug resistance factors in non small cell lung cancer

Objective To study the levels of expression,coexpression and clinical significance of multidrug resistance factors in lung cancer.Methods The expressions of p-glutathione(P-gp),multidrug resistance-associated protein(MRP),glutathione stransferase Ⅱ(GST-Ⅱ) in tumor tissues of 52 lung cancer patients were detected by using immunohistochemical method.Results The positive rates of P-gp,MRP,GST-Ⅱ were 51.9%(27/52),61.5%(32/52),78.8%(41/52),respectively.No relationship was observed between the expression of drug resistance factors and TNM stage and cell differentiation.The coexpression rates were as follows: P-gp+MRP,40.1%;P-gp+GST-Ⅱ,44.2%;MRP+GST-Ⅱ,50.0%;P-gp+MRP+GST-Ⅱ,21.2%;correlations were found between P-gp and MRP(rs=0.632,P<0.01),P-gp and GST-Ⅱ(rs=0.521,P<0.01),MRP and GST-Ⅱ(rs=0.532,P<0.01).Conclusion The multidrug resistance in lung cancer patients is affected by various multidrug resistance factors.The drug resistance factors' expressions are not related to histological subtypes,TNM stage and cell differentiation.