| 日本乙型脑炎病毒单克隆抗体的抗原结合位点及亲和力测定 |
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| 引用本文： | 张明杰,汪美先.日本乙型脑炎病毒单克隆抗体的抗原结合位点及亲和力测定[J].病毒学报,1990,6(1):34-37. |
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| 作者姓名： | 张明杰 汪美先 |
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| 作者单位： | 第四军医大学微生物教研室 西安
(张明杰，汪美先，姜绍谆，马文煜)，第四军医大学微生物教研室 西安(于碧云) |
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| 摘 要： |
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| 关 键 词： | 乙型脑炎病毒 单克隆抗体 抗原位占 |
MEASUREMENT OF ANTIGENIC SITES AND AFFINITY OF THE ANTI-JAPANESE ENCEPHALITIS VIRUS MONOCLONAL ANTIBODIES |
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| Zhang Mingjie Wang Meixan Jiang Shaozhun Ma Wenyu Yu Biyun.MEASUREMENT OF ANTIGENIC SITES AND AFFINITY OF THE ANTI-JAPANESE ENCEPHALITIS VIRUS MONOCLONAL ANTIBODIES[J].Chinese Journal of Virology,1990,6(1):34-37. |
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| Authors: | Zhang Mingjie Wang Meixan Jiang Shaozhun Ma Wenyu Yu Biyun |
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| Abstract: | On the basis of rapid double sandwish enzyme-linked immunoassay (RDS-ELISA), we determined the antigenic sites and relative affinity of the six anti-Japanese encephalitis virus ( JEV ) monoclonal antibodies (McAb). It was found by ELISA additivity test that, the six McAb recognized five different antigenic sites.2H4 and 2F2 recognized the same or very similar antigenic site, but the antigenic sites recognized by the other McAb were different. By analysing the 1/2 maximum binding of the McAb, the relative affinity of the McAb could be ranked as following. nG2>2H4 >2D2>mG9>2F2>mC3, at the protein concentration of 0.01 to 1.50 ng. This study is useful for the applications of the JEV-McAb in immunologi-cal diagnosis and vaccine preparation. |
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| Keywords: | Japanese encephalitis virus Monoclonal antibody Antigenic site Affinity |
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