The Rho Variant-Du |
| |
Authors: | Robert G Pittenger MD Dorothy McQuiston MT Phillip Sturgeon MD Cornelia Gunnink and Jo Ann Agnew |
| |
Affiliation: | American National Red Cross, Pacific Northwest Blood Program, Portland, Oregon, and the National Research Laboratories, Western Division, Los Angeles, California |
| |
Abstract: | A simplified test for the Du factor employing a reagent containing 25 per cent serum albumin, the "stick" technic to introduce cells and forceful cen-trifugation has been given an extensive trial in a routine blood bank operation. A routine preliminary test with anti-D gave 5,051 negative reactions among 26,753 bloods tested. The Du tube test was then performed on the former with an anti-CDE reagent in parallel with the indirect antiglobulin test done with anti-D. All bloods positive by one or both tests were tested further for D antigens by an anti-D elution technic; they were also tested for Rh-Hr factors. Among the 126 bloods positive by elution for the Dufactor, 122 and 126 were positive respectively by the antiglobulin and the Du tube tests. The two tests were in agreement on all eight of the type ccDuee bloods found in the study. The Dutube test, as done with anti-CDE, gave no false negative reactions. It is, thus, concluded that bloods which are negative with this test will be type ccddee and can be labeled "Rh negative tested for Du. Serological factors which might account for sensitivity of the Du tube test's being the equal of the antiglobulin test are considered, as are the probabilities of the existence of D variants so weak that they could not be detected by either test. |
| |
Keywords: | |
|
|