红景天苷对骨髓间充质干细胞增殖的影响

本研究旨在探索红景天苷对骨髓MSC增殖及干细胞因子（SCF）分泌的影响.应用密度梯度离心和贴壁培养法,体外分离并扩增MSC,应用流式细胞术和成脂及成骨诱导法进行MSC鉴定,并观察红景天苷对MSC增殖、细胞周期及分泌SCF的影响.结果表明,红景天苷可影响MSC的增殖,以1.5 mg/ml浓度刺激MSC增殖作用最强,在24、48、72 h内存在一定的时间依赖性,并且红景天苷明显增加S、G1/M期细胞比例,尤以1.5 mg/ml作用明显.应用1.5 mg/ml红景天苷与MSC共培养48 h,其培养上清中SCF含量明显上调（P＜0.01）,其mRNA表达也明显增加（P＜0.01）.结论：在一定剂量浓度下红景天苷对MSC有明显增殖促进作用,并可提高MSC的CSF基因表达和分泌.

Effects of Salidroside on Proliferation of Bone Marrow Mesenchymal Stem Cells

This study was aimed to investigate the effect of salidroside on proliferation of bone marrow mesenchymal stem cells （MSC） and their secretion of stem cell factor （SCF）. MSC were isolated and amplified in vitro via density gradient centrifugation and adherence screening method. MCS were identified by flow cytometry and osteogenic/ adipogenic induction. The effects of salidroside on cell proliferation, cell cycle and the SCF secretion of MSC were detected by flow cytometry. The results showed that the salidroside could induce the proliferation of MSC, peaked at the concentration of 1.5 mg/ml and in a time-dependent manner （in 24 h, 48 h and 72 h）. Salidroside at 1.5 mg/ml could more effectively increase the percentage of cells in S and G∽/M phase. Co-cultured with salidroside at the concentration of 1.5 mg/ml for 48 h, the SCF and the expression levels of SCF mRNA in co-culture supernant were both significantly increased （P 〈 0.01 ）. It is concluded that salidroside in a range of certain concentration can obviously promote the proliferation of MSC and increase the expression and secretion of SCF.