DCE-MRI成像各定量参数对结肠癌裸鼠移植瘤药物治疗疗效的评价

目的利用动态增强磁共振成像技术DCE-MRI成像参数评价抗血管药物抑制肿瘤血管生成的疗效。方法 48只结肠癌裸鼠移植瘤随机分两组,对照组:24只、抗肿瘤药物组:24只,对照组注射等量的0.9%氯化钠溶液、腹腔注射5-氟尿嘧啶(5-FU)0.02ml/天,分别在治疗前与治疗后2天、7天及14天进行常规MRI检查和动态增强检查,治疗后每个时间段各处死6只老鼠行免疫组化染色,与动态增强检查各参数进行相关性分析。结果在DCE-MRI各参数,实验组Ktrans值及Kep值与对照组差异有统计学意义(P0.05),同时间点Ktrans值及同时段的Kep值实验组与对照组比较均有统计学意义(P0.05),并与免疫组化结果做分析,存在良好的相关性。参数值Ve不存在统计学意义。结论裸鼠移植瘤模型提供了一个理想的动物模型研究肿瘤,DCE-MRI成像半定量和定量参数分析肿瘤新生血管生成或抗肿瘤药物治疗后肿瘤血管生成关闭的应用价值。

Evaluation of Colon Cancer Nude Mouse Transplanted Tumor Medication Therapeutic Effect by Various DCE-MRI Imaging Quantitative Parameters

Objective Evaluation of tumor angiogenesis inhibition therapeutic effect by dynamic enhancement DCE-MRI imaging parameters.Methods 48 colon cancer nude mice transplanted tumors were randomly divided into two groups: the control group: 24 mice and the anticancer drug group: 24 mice. The control group of mice had the same amount of 0.9% sodium chloride solution injection and intraperitoneal injection of 5-FU 0.02ml/day, and routine MRI inspection and dynamic enhancement inspection respectively before treatment and 2、7 and 14 days after treatment. In each treatment period after treatment, 6 mice were sacrificed and given immunohistochemical staining, and correlation analysis of which and various dynamic enhancement inspection parameters was made.Results Among DCE-MRI parameters of the anticancer drug group, only Ktrans value and Kep value had their difference with that of the control group of statistical significance (P<0.05). And the contrast between the Ktrans value and Kep value of the 2 groups at the same time point and period was of statistical significance (P<0.05), indicating a good correlation in immunohistochemical analysis. The parameter value Ve was not of statistical significance.Conclusion The nude mice transplanted tumor model is of application value for animal tumor study, analysis of tumor angiogenesis or its closing after antitumor meditation with DCE-MRI imaging semi-quantitative and quantitative parameters.