去势成年雌性大鼠骨质疏松模型血清NO和N0S的水平及其意义

目的 测定大鼠骨质疏松模型血清中NO和NOS的水平,探讨骨质疏松症与NO和NOS浓度的关系。方法 6月龄成年雌性SD大鼠随机分成实验组和对照组,术前及术后均测量腰椎骨密度,术后分离血清,用改良镀铜镉还原法测定NO含量,用化学比色法测定NOS含量。结果 术前,两组大鼠骨密度差异无显著性,术后,实验组大鼠骨密度明显低于对照组骨密度,差异有显著性(P<0.05)。血清NO和NOS水平实验组明显低于对照组,差异有显著性(P<0.05),有统计学意义。结论NO和NOS在骨质疏松的病理生理过程中起重要作用。

Change and significance of serum nitric oxide and nitric oxide synthase in adult ovariectomy rats modle of osteoorosis

Objective To measure the serum levels of nitric oxide (NO) and nitric oxide synthase (NOS) in adult ovariectomized rat model of osteoporosis (OP) and study the relationship between NO, NOS levels and os-teoporosisn.Methods 6-month-old adult female SD rats were divided at radom into ovariectomized osteoporotic group and control group. Bone mineral density (BMD) was measured pre-and post-operation and serum was sepa-rated after operation, the was assayed NO content by modified plated copper cadmium reduction and for NOS con-tent by chemical colorimetry. Results There was significant difference in BMD before operation. After operation, howere, BMD and NO, NOS levels in the model group were far lower than those in the control group, with a sig-nificant difference (P < 0.05). Conclusion NO and NOS play an important role in the pathophysiological course of osteoporosis.