| 藏灵菇源干酪乳杆菌KL1胞外多糖抑制人结肠癌细胞增殖的研究 |
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| 引用本文： | 邢书涵,连正兴,孙勇,韩红兵,钟思琼,刘慧.藏灵菇源干酪乳杆菌KL1胞外多糖抑制人结肠癌细胞增殖的研究[J].食品科学,2012(7):284-288. |
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| 作者姓名： | 邢书涵 连正兴 孙勇 韩红兵 钟思琼 刘慧 |
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| 作者单位： | 北京农学院食品科学与工程学院;中国农业大学动物科技学院,农业生物技术国家重点实验室;北京市食品研究所;华中农业大学食品科学技术学院 |
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| 基金项目： | 北京市自然科学基金项目(5092008;5062006);北京市重点建设学科资助项目(PXM2009_014207_078172) |
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| 摘 要： | 分离纯化藏灵菇源干酪乳杆菌KL1胞外多糖(EPS),研究EPS纯品对HCT-8人结肠癌细胞的增殖抑制和诱导细胞凋亡作用。利用Sepharose CL-6B凝胶柱探讨干酪乳杆菌KL1菌株EPS粗品的纯化条件,应用紫外全波长扫描及苯酚-硫酸法鉴定EPS纯度;采用CCK-8法和Annexin V-FITC细胞凋亡检测试剂盒研究EPS对HCT-8人结肠癌细胞的增殖抑制和凋亡作用。结果表明:在0.02～0.12mol/L磷酸盐缓冲液梯度洗脱、流速3mL/min、样品上样质量浓度15.0mg/mL、样品上样量2.0mL的纯化条件下获得EPSa和EPSb两个单一组分的胞外多糖,其纯度分别为91.67%和82.86%。EPSa处理HCT-8人结肠癌细胞体外实验发现,EPSa对HCT-8细胞的增殖有抑制作用,以及诱导细胞凋亡的发生,且细胞生长抑制率呈时间-剂量依赖性。提示藏灵菇源干酪乳杆菌KL1菌株的EPSa有抑制结肠癌细胞增殖的生物活性。
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| 关 键 词： | 干酪乳杆菌 胞外多糖 HCT-8人结肠癌细胞 抑制增殖 细胞凋亡 |
Inhibitory Effect of Exopolysaccharides Produced by Lactobacillus casei Strain KL1 from Tibetan Kefir on Proliferation of Human Colon Cancer Cells |
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| XING Shu-han,LIAN Zheng-xing,SUN Yong,HAN Hong-bing,ZHONG Si-qiong,LIU Hui.Inhibitory Effect of Exopolysaccharides Produced by Lactobacillus casei Strain KL1 from Tibetan Kefir on Proliferation of Human Colon Cancer Cells[J].Food Science,2012(7):284-288. |
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| Authors: | XING Shu-han LIAN Zheng-xing SUN Yong HAN Hong-bing ZHONG Si-qiong LIU Hui |
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| Affiliation: | 1,(1.College of Food Science and Engineering,Beijing University of Agriculture,Beijing 102206,China;2.State Key Laboratorys for Agro-biotechnology,College of Animal Science and Technology,China Agricultural University,Beijing 100094,China;3.Beijing Food Research Institute,Bejing 100162,China;4.College of Food Science and Technology,Huazhong Agricultural University,Wuhan 430070,China) |
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| Abstract: | In this study,exopolysaccharides(EPS) produced by Lactobacillus casei strain KL1,isolated from Tibetan Kefir,were purified and used to explore the effect on the proliferation of human colon cancer HCT-8 cells.The EPS was purified by Sepharose CL-6B gel column and its purity was evaluated by UV scanning spectroscopy and phenol-sulfuric acid assay.The cell proliferation and apoptotic effect of EPS on HCT-8 cells was examined by CCK-8 assay and Annexin V-FITC apoptosis detection kit.Two individual components(EPSa and EPSb) were obtained under the optimal purification conditions: gradient elution with 0.02-0.12 mol/L phosphate buffer at a flow rate of 3 mL/min,and sample concentration of 15.0 mg/mL with a loading volume of 2.0 mL.The purities of EPSa and EPSb were 91.67% and 82.86%,respectively.EPSa could inhibit HCT-8 cell proliferation and induce cell apoptosis.The inhibitory rate of cell growth was in a dose-dependent manner.Therefore,EPSa reveals therapeutic effect on colon cancer. |
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| Keywords: | Lactobacillus casei exopolysaccharides HCT-8 human colon cancer cell cell proliferation inhibition cell apoptosis |
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